Measuring serum total and free indoxyl sulfate and p-cresyl sulfate in chronic kidney disease using UPLC-MS/MS

Publication date: Available online 4 December 2018Source: Journal of Food and Drug AnalysisAuthor(s): Chia-Ni Lin, I-Wen Wu, Yun-Fen Huang, Shu-Yu Peng, Ya-Ching Huang, Hsiao-Chen NingAbstractChronic kidney disease (CKD) is a complex disorder that affects multiple organs and increases the risk of cardiovascular complications. CKD affects approximately 12% of the population in Taiwan. Loss of kidney function leads to accumulation of potentially toxic compounds such as indoxyl sulfate (IS) and p-cresyl sulfate (pCS), two protein-bound uremic solutes that can stimulate the progression of CKD. The aim of this study was to assess whether IS and pCS levels were correlated with CKD stage. We developed and validated a method for quantitating total and free IS and pCS in serum by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Serum samples were pretreated using protein precipitation with acetonitrile containing stable isotope-labeled IS and pCS as internal standards. After centrifugation, the supernatant was diluted and injected into a UPLC-MS/MS system. Analyte concentrations were calculated from the calibration curve and ion ratios between the analyte and the internal standard. The calibration curves were linear with a correlation coefficient of>0.999; the analytical measurement range was 0.05–5 mg/L. The limit of quantitation of this assay was 0.05 mg/L for both analytes. The reference interval was ≤0.05–1.15 mg/L for...
Source: Journal of Food and Drug Analysis - Category: Food Science Source Type: research

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PMID: 30994543 [PubMed - in process]
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Source: Kidney International - Category: Urology & Nephrology Authors: Tags: Commentary Source Type: research
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Source: Kidney International - Category: Urology & Nephrology Authors: Tags: Commentary Source Type: research
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Source: Kidney International - Category: Urology & Nephrology Authors: Tags: KDIGO Executive Conclusions Source Type: research
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Conditions:   Diabetes Mellitus, Type 2;   Renal Insufficiency, Chronic Interventions:   Drug: Metformin;   Drug: Sulfonylurea;   Drug: DPP-4 inhibitor;   Drug: SGLT2 inhibitor;   Drug: GLP1 receptor agonist Sponsors:   Weill Medical College of Cornell University;   Patient-Centered Outcomes Research Institute Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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