Geometric, elastic and contractile-relaxation changes in coronary arterioles induced by Vitamin D deficiency in normal and hyperandrogenic female rats

Publication date: Available online 28 November 2018Source: Microvascular ResearchAuthor(s): Leila Hadjadj, Anna Monori-Kiss, Eszter M. Horváth, Andrea Heinzlmann, Attila Magyar, Réka E. Sziva, Zsuzsanna Miklós, Éva Pál, János Gál, István Szabó, Zoltán Benyó, György L. Nádasy, Szabolcs VárbíróAbstractVitamin D (VitD) hypovitaminosis and androgen excess (AE) are both risk factors for cardiovascular diseases in fertile women. However, the possible early interaction between AE and VitD status is not clear. Our goal was to describe how VitD status influences early changes in the biomechanical reactivity of small coronary arterioles in adult female rats after transdermal testosterone treatment.Forty-six adolescent, 90–110-gram-weighed female Wistar rats were randomly grouped into 4 groups. Twenty-four animals received an optimal VitD-supplemented diet, from which 12 animals underwent transdermal testosterone treatment. Twenty-two animals received a VitD-deficient diet, from which 11 were treated with testosterone. At 8 weeks of treatment, invasive arterial blood pressure was registered after in vivo cannulation of carotid artery. Arteriolar end and side branches (200 μm diameter) of the left anterior descendent coronary artery (LAD) were obtained and examined with pressure arteriography in vitro. Similar segments were removed for histological examination. The inner and outer radii of the arterioles were measured using video-microscopy. Normal myogenic tone, m...
Source: Microvascular Research - Category: Biochemistry Source Type: research