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YAP1-TEAD1 signaling controls angiogenesis and mitochondrial biogenesis through PGC1 α
Publication date: Available online 20 April 2018 Source:Microvascular Research Author(s): Akiko Mammoto, Megan Muyleart, Andrew Kadlec, David Gutterman, Tadanori Mammoto Mitochondria contribute to key processes of cellular function, while mitochondrial dysfunction is implicated in metabolic disorders, neurodegenerative diseases, and cardiovascular diseases, in which angiogenesis - the formation of new blood capillaries - is dysregulated. The Hippo signaling transducer, Yes-associated protein (YAP1) binds to the TEA domain (TEAD1) transcription factor and controls angiogenesis. YAP1 also regulates glucose metabolism throug...
Source: Microvascular Research - April 20, 2018 Category: Biochemistry Source Type: research

Statins reduce testicular and ocular VEGF: A potential compromise to microcirculation
Conclusion Statin use decreased in VEGF levels of testicular and ocular tissues in diabetic and non-diabetic rats. Statin treatment (anti-VEGF effect) had a protective effect in the development of diabetic retinopathy, yet statins may have a negative impact on tissues that depend on microcirculation by reducing VEGF levels. Further research is needed for statins' microcellular effects. (Source: Microvascular Research)
Source: Microvascular Research - April 18, 2018 Category: Biochemistry Source Type: research

Interaction between blood and solid particles propagating through a capillary with slip effects
This article describes the interaction between solid particles and blood propagating through a capillary. A slip condition is considered on the walls of the capillary. The rheological features of the blood are discussed by considering as a two-phase Newtonian fluid model, i.e., the suspension of cells in plasma. A perturbation method is successfully applied to obtain the series solution of the governing coupled differential equations. The series solution for both fluid and particle phase are presented up to second order approximation. The expressions for the velocity and pressure distributions under slip effects are determ...
Source: Microvascular Research - April 18, 2018 Category: Biochemistry Source Type: research

The effect of repeated bouts of hyperaemia on sensory nerve-mediated cutaneous vasodilatation in humans
Conclusions Sensory nerve blockade attenuated the improvements in cutaneous vascular responses to thermal hyperaemia and PORH following chronic exposure to shear stress. These data demonstrate an important role for sensory nerve function in the initiation of vasodilatation to both PORH and thermal hyperaemia, in both the time to onset and the magnitude of vasodilatation. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Anti-angiogenic effect of a humanized antibody blocking the Wnt/ β-catenin signaling pathway
Conclusions H1L1 exhibits anti-angiogenic activities through blocking the Wnt/β-catenin pathway. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Nitric oxide signaling regulates tumor-induced intussusceptive-like angiogenesis
In this study, we developed an ex-ovo egg yolk angiogenesis model to screen the angiogenic potency of tumor cells (HeLa and SiHa cell lines). The egg yolk angiogenesis assay was applied to study the nitric oxide (NO) influence on switching from sprouting angiogenesis (SA) to intussusceptive angiogenesis (IA) under tumor microenvironment. Morphological analysis and SA-like or IA-like markers expression were determined during the development of chicken chorioallantoic membrane (CAM) from day 5 to 13. Expression of Notch1, Notch2, EphrinB2, and Tie2 were considered as SA-like while TEM8, CALD1, CXCR4 and HOMX1 were followed a...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Numerical modeling of fluid and oxygen exchanges through microcirculation for the assessment of microcirculation alterations caused by type 2 diabetes
This study illustrates that when microcirculation disturbances, including the structure of capillary network, the wall osmosis property and the capacity of blood binding oxygen occur in DM2, some negative impacts are raised on microvascular hemodynamics and metabolism circumstance of interstitial tissue. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Sitting time is negatively related to microvascular endothelium-dependent function in rheumatoid arthritis
Conclusion Habitual sedentary behaviour (sitting time) appears to be adversely linked to microvascular endothelium-dependent function among people living with RA. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Reproducibility and agreement of different non-invasive methods of endothelial function assessment
In conclusion, the LTH plateau response is for two-third NO-dependent, but unrelated to FMD. Furthermore, despite easy to perform the LTH responses are not more reproducible than FMD. Given the weak associations, the different methods of vascular function assessment are not interchangeable. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Automated structure and flow measurement — a promising tool in nailfold capillaroscopy
Conclusions Structural and blood flow measurements are both able to distinguish patients with SSc from those with PRP/HC. Importantly, these hold promise as clinical trial outcome measures for treatments aimed at improving finger blood flow or microvascular remodelling. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Numerical simulation of heat transfer in blood flow altered by electroosmosis through tapered micro-vessels
This study is encouraged by exploring the nanofluid-dynamics in peristaltic transport as symbolized by heat transport in biological flows and also in novel pharmacodynamics pumps and gastro-intestinal motility enhancement. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Microvascular abnormalities in dry eye patients
This study was conducted to evaluate conjunctival blood flow velocities and microvascular network density in patients with dry eye disease (DED). Twenty-five patients with DED and 25 healthy controls were recruited. The microvasculature and microcirculation of the temporal bulbar conjunctiva of the right eyes were assessed using a functional slit-lamp biomicroscope. Vascular variables included blood flow velocity (BFV), blood flow rate (BFR), microvascular network density and vessel diameter. A fractal analysis was performed using the box counting method to measure the fractal dimension (Dbox) representing the vessel densi...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Isolating the influences of fluid dynamics on selectin-mediated particle rolling at venular junctional regions
The objective of this study was to isolate the impact of hydrodynamics on selectin-mediated cell rolling in branched microvessels. Significant advancements have been made in furthering the understanding of complex interactions between biochemical and physical factors in the inflammatory cascade in simplified planar geometries. However, few studies have sought to quantify the effects of branched configurations and to isolate the effects of associated fluid forces. Experimental techniques were developed to perform in vitro adhesion experiments in Y-shaped micro-slides. The micro-slides were coated with P-selectin and microsp...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Acute alterations in the hematological and hemorheological profile induced by resistance training and possible implication for microvascular functionality
Publication date: July 2018 Source:Microvascular Research, Volume 118 Author(s): Daniel A. Bizjak, Daniel Jacko, Philipp Zimmer, Sebastian Gehlert, Wilhelm Bloch, Marijke Grau Depending on the exercise variables and training design, resistance exercise can be applied to gain muscle mass, prevent diseases like osteoporosis and sarcopenia or generally increase strength capacity. But the influence on blood flow parameters and possible consequences in health and disease are less understood. To examine the possible impact of resistance exercise of different duration on hemorheology, oxidative stress and microvascular function,...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Vascular architecture in free flaps: Analysis of vessel morphology and morphometry in murine free flaps
Publication date: July 2018 Source:Microvascular Research, Volume 118 Author(s): Andreas Max Pabst, Peer Wolfgang Kämmerer, Maximilian Krüger, Lukas Jäger, Maximilian Ackermann The aim of this study was to analyze the development of vascular architecture as well as vascular morphometry and morphology of anastomosed microvascular free flaps. Free pectoral skin flaps were raised in 25 rats and anastomosed to the femoral vessels in the groin region. CD31 immunohistology was performed after 3, 7 and 12 d (each 5 animals each) to analyze microvessel density (MVD), microvessel area (MVA) and microvessel size (M...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Retinal capillary and arteriolar changes in patients with chronic kidney disease
Conclusion Patients with moderately severe CKD show retinal signs of end-organ damage indicated by an increased wall-to-lumen ratio and capillary rarefaction. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

The emerging application of semi-quantitative and quantitative capillaroscopy in systemic sclerosis
Publication date: July 2018 Source:Microvascular Research, Volume 118 Author(s): Carina Mihai, Vanessa Smith, Rucsandra Dobrota, Ana Maria Gheorghiu, Maurizio Cutolo, Oliver Distler Systemic sclerosis (SSc) is a connective tissue disease with high morbidity and mortality, characterized by autoimmunity, obliterative vasculopathy involving mainly the microvasculature, and fibrosis. SSc-specific nailfold capillaroscopic changes have been defined, and nailfold capillaroscopy (NFC) is now unequivocally accepted to be a cornerstone for the early diagnosis of SSc. However, the use of NFC in patients already diagnosed with SSc is...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Generation of human umbilical cord vein CD146+ perivascular cell origined three-dimensional vascular construct
In conclusion, by combining natural scaffolds and vascular cells differentiated from CD146+ PCs, VCs can be generated layer by layer. This study will provide a preliminary blood vessel model for generation of fully natural small-diameter vascular grafts. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

High glucose induces dysfunction of human umbilical vein endothelial cells by upregulating miR-137 in gestational diabetes mellitus
Publication date: July 2018 Source:Microvascular Research, Volume 118 Author(s): Hai-Yan Peng, Hua-Ping Li, Ming-Qing Li Recent studies have revealed considerable dysfunction of vascular endothelial cells (VECs) and abnormal expression of microRNA (miR)-137 in women with gestational diabetes mellitus (GDM), and the aim of this study was to clarify the underlying mechanism and possible role of microRNA (miR)-137 in dysfunction of VECs during GDM. We found increased levels of miR-137 in the plasma of GDM women and high-glucose (HG)-exposed HUVECs. Upregulating miR-137 in HUVECs elevated the chemokine (C-C motif) ligand 2 (C...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Protective effects of saffron and its active components against oxidative stress and apoptosis in endothelial cells
In this study, we investigated the role of mitogen-activated protein kinase (MAPK) signaling pathways in mediation of the protective effects of saffron extract, saffron essential oil, safranal and crocin on bovine aortic endothelial cells against oxidative injury. The viability of cells in response to H2O2-induced toxicity (0.4, 2 and 10 mM) was measured using resazurin assay in the presence or absence of saffron extract (2–40 μg/ml), saffron oil (2–40 μg/ml), safranal (2–40 μM) and crocin (2–40 μM). Dichlorodihydrofluorescin diacetate was used as an indicator for the amount of...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Influence of microvascular sutures on shear strain rate in realistic pulsatile flow
This study, for the first time, investigates the influence of pulsatile flow on local haemodynamics within sutured microarterial anastomoses. We measured physiological arterial waveform velocities experimentally using Doppler ultrasound velocimetry, and a representative example was applied to a realistic sutured microarterial geometry. Computational geometries were created using measurements taken from sutured chicken femoral arteries. Arterial SSRs were predicted using computational fluid dynamics (CFD) software, to indicate the potential for platelet activation, deposition and thrombus formation. Predictions of steady an...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Evaluation of microvascular endothelial function and capillary density in patients with infective endocarditis using laser speckle contrast imaging and video-capillaroscopy
Conclusions The main findings in the microcirculation of patients with IE were greater basal vasodilation and a reduction of the endothelium-dependent and -independent microvascular reactivity, as well as greater functional skin capillary density compared to healthy individuals. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

A new role of mast cells in arteriogenesis
Publication date: July 2018 Source:Microvascular Research, Volume 118 Author(s): Domenico Ribatti Arteriogenesis is defined as the growth of functional collateral arteries from pre-existing arterio-arteriolar anastomoses. The role of mast cells in arteriogenesis is largely unexplored. Recent evidences suggest that mast cells together with other inflammatory cells, including monocytes-macrophages, lymphocytes, NK cells and endothelial precursor cells (EPCs) may be involved in this process. This review article analyzes the literature concerning this new aspect of biological activity of mast cells. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Hyperbilirubinemia-induced pro-angiogenic activity of infantile endothelial progenitor cells
Conclusions Our results showed that hyperbilirubinemia can activate migration, proliferating and angiogenic properties of cEPCs. Hyperbilirubinemia can promote the proangiogenic secretory activity of cEPCs, thereby resulting in enhancement of their regenerative wound healing properties. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Alterations of conjunctival glycocalyx and microcirculation in non-septic critically ill patients
Publication date: July 2018 Source:Microvascular Research, Volume 118 Author(s): Andrius Pranskunas, Tomas Tamosuitis, Neringa Balciuniene, Diana Damanskyte, Edvin Sneider, Astra Vitkauskiene, Edmundas Sirvinskas, Vidas Pilvinis, E. Christiaan Boerma As of now the relationship between glycocalyx degradation and microcirculatory perfusion abnormalities in non-septic critical ill patients is unclear. In addition, conjunctival sidestream dark field-imaging for the purpose of glycocalyx thickness estimation has never been performed. We aimed to investigate whether changes in glycocalyx thickness in non-septic patients are ass...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Fractal dimension and lacunarity analysis of retinal microvascular morphology in hypertension and diabetes
Publication date: July 2018 Source:Microvascular Research, Volume 118 Author(s): Natasa Popovic, Miroslav Radunovic, Jelena Badnjar, Tomo Popovic Hypertension and diabetes mellitus represent modifiable risk factors for vascular disease. They cause microvascular remodeling, and ultimately result in end-organ damage. Therefore, development of methods for noninvasive quantification of the effects of hypertension and diabetes mellitus on microvasculature is of paramount importance. The two goals of the study were: 1) to characterize the geometric complexity and inhomogeneity of retinal vasculature in hypertensive retinopathy ...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Near-infrared spectroscopy assessment of microvasculature detects difference in lower limb vascular responsiveness in obese compared to lean individuals
Conclusion This study demonstrated differences in vascular responses within the microvasculature of the lower limb between lean and obese individuals. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Modeling of FMISO [F18] nanoparticle PET tracer in normal-cancerous tissue based on real clinical image
Publication date: July 2018 Source:Microvascular Research, Volume 118 Author(s): Hanie Asgari, M. Soltani, Mostafa Sefidgar Hypoxia as one of the principal properties of tumor cells is a reaction to the deprivation of oxygen. The location of tumor cells could be identified by assessment of oxygen and nutrient level in human body. Positron emission tomography (PET) is a well-known non-invasive method that is able to measure hypoxia based on the FMISO (Fluoromisonidazole) tracer dynamic. This paper aims to study the PET tracer concentration through convection–diffusion-reaction equations in a real human capillary-like...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Ischemia-reperfusion injury alters skin microvascular responses to local heating of the index finger
Conclusions The current IR model elicits impaired cutaneous vasodilatory responses to local heating in young males, primarily in non-glabrous skin, and may be useful for exploring mechanisms of IR-injury and for testing potential countermeasures in otherwise healthy humans. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Inter-visit variability of conjunctival microvascular hemodynamic measurements in healthy and diabetic retinopathy subjects
Publication date: July 2018 Source:Microvascular Research, Volume 118 Author(s): Maziyar M. Khansari, Michael Tan, Preny Karamian, Mahnaz Shahidi Conjunctival microcirculation imaging provides a non-invasive means for detecting hemodynamic alterations due to systemic and ocular diseases. However, reliable longitudinal monitoring of hemodynamic changes due to disease progression requires establishment of measurement variability over time. The purpose of the current study was to determine inter-visit variability of conjunctival microvascular hemodynamic measurements in non-diabetic control (NC, N = 7) and diabetic retin...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Attenuated cutaneous microvascular function in healthy young African Americans: Role of intradermal l-arginine supplementation
Publication date: July 2018 Source:Microvascular Research, Volume 118 Author(s): Kiyoung Kim, Chansol Hurr, Jordan C. Patik, R. Matthew Brothers It has been established that endothelial function in conduit vessels is reduced in young African Americans (AA) relative to Caucasian Americans (CA). However, less is known regarding endothelial function in microvasculature of young AA. We hypothesized that microvascular function in response to local heating of skin is attenuated in young AA relative to age-matched CA due largely to the lack of NO bioavailability, which is in turn improved by intradermal l-arginine supplementatio...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Homocysteine up-regulates ETB receptors via suppression of autophagy in vascular smooth muscle cells
In conclusion, this suggested that Hcy up-regulated ETB receptors by inhibiting autophagy in VSMCs via AMPK/mTOR signaling pathway. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Endothelial dysfunction, endothelial nitric oxide bioavailability, tetrahydrobiopterin, and 5-methyltetrahydrofolate in cardiovascular disease. Where are we with therapy?
Publication date: September 2018 Source:Microvascular Research, Volume 119 Author(s): Matthew F. Yuyun, Leong L. Ng, G. André Ng Homeostasis around vascular endothelium is a function of the equilibrium between the bioavailability of nitric oxide (NO) and oxidizing reactive oxygen species (ROS). Within the vascular endothelium, NO enhances vasodilatation, reduces platelet aggression and adhesion (anti-thrombotic), prevents smooth muscle proliferation, inhibits adhesion of leukocytes and expression of pro-inflammatory cytokines genes (anti-inflammatory), and counters the oxidation of low density lipoprotein (LDL) cho...
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Circulating CD34+ and CD34+VEGFR2+ progenitor cells are associated with KLOTHO KL-VS polymorphism
Conclusion One of the mechanisms that are responsible for previously described KL-VS heterozygote advantage may be connected with maintaining greater size of hematopoietic and endothelial progenitor cells population. (Source: Microvascular Research)
Source: Microvascular Research - April 13, 2018 Category: Biochemistry Source Type: research

Intra-test and test-retest reliability of exercise oximetry in arterial claudication
Conclusions Intra-test and test-retest reliability is excellent using the DROP calculation for exercise-tcpO2 investigations. (Source: Microvascular Research)
Source: Microvascular Research - January 9, 2018 Category: Biochemistry Source Type: research

Hyperglycemia induced early growth response-1 regulates vascular dysfunction in human retinal endothelial cells
This study aims at deciphering its role in hyperglycemia induced changes of retinal endothelium. Human retinal endothelial cells (hRECs) were exposed to hyperglycemia (25mM) and normoglycemia (5.5mM). Gene silencing was done using siRNA against Egr-1. Transcript and protein level analysis of Egr-1 and gene targets were done using qPCR and immunoblotting respectively in hRECs, diabetic and nondiabetic human retina and immunofluorescence for localization in retinal sections. Hyperglycemia induced Egr-1 and vascular endothelial growth factor-A (VEGF-A) but not pigment epithelium derived factor (PEDF) in hRECs. Expression of E...
Source: Microvascular Research - January 4, 2018 Category: Biochemistry Source Type: research

Study of microvascular non-Newtonian blood flow modulated by electroosmosis
This study reveals that blood flow rate accelerates with decreasing the plug width (i.e. viscoplastic nature of fluids) and also with increasing the Debye length parameter. (Source: Microvascular Research)
Source: Microvascular Research - January 3, 2018 Category: Biochemistry Source Type: research

Joule heating and zeta potential effects on peristaltic blood flow through porous micro vessels altered by electrohydrodynamic
Publication date: Available online 29 December 2017 Source:Microvascular Research Author(s): N.K. Ranjit, G.C. Shit, D. Tripathi In most of the medical therapies, electromagnetic field plays important role to modulate the blood flow and to reduce the pain of human body. With this fact, this paper presents a mathematical model to study the peristaltic blood flow through porous microvessels in presence of electrohydrodynamics. The effects of Joule heating and different zeta potential are also considered. Darcy law is employed for porous medium. The mathematical analysis is carried out in the form of electroosmosis, flow ana...
Source: Microvascular Research - December 29, 2017 Category: Biochemistry Source Type: research

Sensibility and specificity of laser speckle contrast imaging according to Endo-PAT index in type 1 diabetes
Conclusion In the present study, we could find an ideal cut off value of microcirculation assessment through endothelium-dependent vasodilation to ACh using LSCI according to Endo-PAT's score, only in those under 5years of disease duration. Further prospective studies shall be conducted to evaluate its predictive cardiovascular value. (Source: Microvascular Research)
Source: Microvascular Research - December 16, 2017 Category: Biochemistry Source Type: research

Voltage-gated potassium channels and NOS contribute to a sustained cutaneous vasodilation elicited by local heating in an interactive manner in young adults
In this study, we tested the hypothesis that voltage-gated potassium (Kv) channels contribute to the plateau of the cutaneous vasodilation during local heating through NOS-dependent mechanisms. Eleven young males (25±4years) participated in this study wherein cutaneous vascular conductance (CVC) was measured at four intradermal microdialysis sites that were continuously perfused with either 1) lactated Ringer (Control), 2) 10mM 4-aminopyridine (Kv channel blocker), 3) 10mM Nω-Nitro-L-arginine (NOS inhibitor), or 4) a combination of 4-aminopyridine and Nω-Nitro-L-arginine. In comparison to the Control sit...
Source: Microvascular Research - December 16, 2017 Category: Biochemistry Source Type: research

Developmental changes of l-arginine transport at the blood-brain barrier in rats
Publication date: Available online 14 December 2017 Source:Microvascular Research Author(s): Masanori Tachikawa, Shirou Hirose, Shin-ichi Akanuma, Ryo Matsuyama, Ken-ichi Hosoya l-Arginine is required for regulating synapse formation/patterning and angiogenesis in the developing brain. We hypothesized that this requirement would be met by increased transporter-mediated supply across the blood-brain barrier (BBB). Thus, the purpose of this work was to test the idea that elevation of blood-to-brain l-arginine transport across the BBB in the postnatal period coincides with up-regulation of cationic acid transporter 1 (CAT1) ...
Source: Microvascular Research - December 16, 2017 Category: Biochemistry Source Type: research

Important signals regulating coronary artery angiogenesis
Publication date: May 2018 Source:Microvascular Research, Volume 117 Author(s): Sara Shoeibi, Paul Mozdziak, Shabnam Mohammadi Angiogenesis is a complex process of budding, the formation of new blood vessels from pre-existing microvessels, via migration, proliferation and survival. Vascular angiogenesis factors include different classes of molecules that have a fundamental role in blood vessel formation. Numerous inducers of angiogenesis, such as the members of the vascular endothelial growth factor (VEGF) family, basic fibroblast growth factor (bFGF), angiopoietin (Ang), hepatocyte growth factor (HGF), and hypoxia induci...
Source: Microvascular Research - December 16, 2017 Category: Biochemistry Source Type: research

Endothelial and kidney function in women with a history of preeclampsia and healthy parous controls: A case control study
Conclusion At least 4years after pregnancy, women with a history of PE demonstrated decreased flow mediated dilatation when compared to healthy parous controls. In this study, decreased flow mediated dilation however did not coincide with decreased kidney function. (Source: Microvascular Research)
Source: Microvascular Research - November 8, 2017 Category: Biochemistry Source Type: research

Hyperspectral imaging for monitoring of perfusion failure upon microvascular anastomosis in the rat hind limb
Conclusions The TIVITA™ camera system is a valid non-invasive tool to assess tissue perfusion after microvascular anastomosis. As it safely shows problems in oxygenation, it allows the clinician a determined revision of the site in time in order to prevent prolonged ischemia. (Source: Microvascular Research)
Source: Microvascular Research - November 6, 2017 Category: Biochemistry Source Type: research

Impact of shear rate pattern on post-occlusive near-infrared spectroscopy microvascular reactivity
Publication date: March 2018 Source:Microvascular Research, Volume 116 Author(s): M. Ramos Gonzalez, J.T. Caldwell, P.A. Branch, G.C. Wardlow, C.D. Black, J. Campbell, R.D. Larson, C.J. Ade The primary aim of the present study was to determine the impact of acute changes in shear rate patterns, in particular retrograde shear rate, on microvascular function in 15 healthy, young men and women as determined via the post-occlusive near-infrared spectroscopy (NIRS) microvascular reactivity response. Microvascular reactivity, via NIRS-derived measurements of post-occlusion tissue saturation index (TSI%) and total microvascular ...
Source: Microvascular Research - November 6, 2017 Category: Biochemistry Source Type: research

In vitro characteristics of endothelial cells prepared from human cerebral arteriovenous malformation lesions using a novel method
Conclusion Using the protocol, isolated cAVM-ECs are stably established, and retain their endothelial phenotypes. These cAVM-ECs may provide a biological tool to examine molecular phenotypes and mechanisms responsible for human cAVM. (Source: Microvascular Research)
Source: Microvascular Research - October 28, 2017 Category: Biochemistry Source Type: research

Blood flow, volume and arterio-venous passages in induced mammary tumours of the rat
Conclusion In induced rat mammary tumours, a high fraction of blood, 28%, passes arterio-venous vessels between 15 and 25μm and there also exist passages >25μm. These findings indicate that the functional capacity of the tumour vascular bed might be impaired, adding to the abnormal microenvironment of tumours. (Source: Microvascular Research)
Source: Microvascular Research - October 22, 2017 Category: Biochemistry Source Type: research

Angiotensin II regulates δ-ENaC in human umbilical vein endothelial cells
In this study we report the presence of δ ENaC in HUVECS with a half-life of ~80min and a change in δ ENaC abundance when HUVECs were treated with angiotensin II. We also observed that angiotensin II increased apical membrane expression of δ ENaC and decreased protein ubiquitination. Equivalent short circuit current measurements showed angiotensin II increased δ ENaC ion transport in HUVEC cells. Treatment with the antioxidant apocynin attenuated angiotensin II mediated effects indicating an important role for angiotensin-derived H2O2 in δ ENaC subunit regulation. Whole cell recordings from oo...
Source: Microvascular Research - October 20, 2017 Category: Biochemistry Source Type: research

Unraveling the role of hypoxia-inducible factor (HIF)-1 α and HIF-2α in the adaption process of human microvascular endothelial cells (HMEC-1) to hypoxia: Redundant HIF-dependent regulation of macrophage migration inhibitory factor
Publication date: Available online 6 October 2017 Source:Microvascular Research Author(s): Martin Hahne, Peggy Schumann, Mathias Mursell, Cindy Strehl, Paula Hoff, Frank Buttgereit, Timo Gaber Hypoxia driven angiogenesis is a prominent feature of tissue regeneration, inflammation and tumor growth and is regulated by hypoxia-inducible factor (HIF)-1 and -2. The distinct functions of HIFs in the hypoxia-induced angiogenesis and metabolic switch of endothelial cells are still unknown and therefore aim of this study. We investigated the role of HIF-1 and -2 in the adaptation of immortalized human microvascular endothelial cel...
Source: Microvascular Research - October 7, 2017 Category: Biochemistry Source Type: research

Effects of insulin analogs as an add-on to metformin on cutaneous microcirculation in type 2 diabetic patients
Conclusions A strategy of improving glycemic control for 4weeks with insulin analogs improves microcirculatory endothelial reactivity and reduces endothelial biomarkers at fasting, whatever the insulin regimen used. Insulin therapy associated to metformin is able to improve fasting microvascular endothelial function even before complete metabolic control. (Source: Microvascular Research)
Source: Microvascular Research - September 25, 2017 Category: Biochemistry Source Type: research