Reduced SIRT1/AMPK in Amyotrophic Lateral Sclerosis Patient ‐Derived Mesenchymal Stem Cells can be restored by resveratrol

In this study, we examined SIRT1/AMPK activities and identified significant decreases in the ALS‐MSCs compared to normal healthy control origi nated BM‐MSCs. This decline was restored by pre‐treatment with resveratrol (RSV), measured using quantitative PCR, NAD/NADH assay, and immunoblot analysis. Neuro‐progenitor markers were increased in RSV treated ALS‐MSCs (RSV/ALS‐MSCs). The differentiated ALS‐MSCs exhibited a cell body an d dendritic shape similar to neurons. RSV/ALS‐MSCs showed significantly increased differentiation rate as compared to the untreated differentiated ALS‐MSCs (ALS‐dMSCs). The neurite numbers and lengths were also significantly increased. This was confirmed with immunoblot analysis using neuron s pecific markers such as nestin, NF‐M, Tuj‐1, and Map‐2 in RSV/ALS‐dMSCs. Thus, this study shows that ALS‐MSCs showed down‐regulation of AMPK/SIRT1 signaling, which was recovered by treatment with RSV. This data suggest that RSV can be one of candidate agents for improving therapeutic eff icacy of ALS patients’ originated MSCs.
Source: Journal of Tissue Engineering and Regenerative Medicine - Category: Biotechnology Authors: Tags: RESEARCH ARTICLE Source Type: research