Malondialdehyde Assay in the Evaluation of Aspirin Antiplatelet Effects

In this study, we compared this MDA assay to the conventional assays in determination of pharmacodynamic aspirin response. For this, aspirin antiplatelet effects were measured in 22 healthy individuals and 63 aspirin treated patients using TX B2 formation enzyme-linked antibody assay, arachidonic acid induced light transmission aggregometry (LTA) and the new fluorometric MDA assay. In patients, MDA levels correlated well with TX formation (R = 0.81; 95% CI 0.69 –0.88;p #x3c; 0.001) and LTA (R = 0.84; CI 0.74 –0.91;p #x3c; 0.001). Receiver operating characteristic analyses revealed that the MDA assay does detect HTPR to aspirin sufficiently (area under the curve: 0.965;p #x3c; 0.001). The optimal cut-off was #x3e; 128 nmol/L (sensitivity of 100%, specificity of 91%). The new MDA assay is reliable in detecting HTPR. It is highly specific in the evaluation of antiplatelet effects by aspirin. This promising and potential bedside assay needs to be evaluated in clinical practice.Pharmacology 2019;103:23 –29
Source: Pharmacology - Category: Drugs & Pharmacology Source Type: research