Sterol regulatory element binding protein-1: Molecular cloning, tissue distribution and gene expression level in response to nutritional regulation in mud crab, Scylla paramamosain.

Sterol regulatory element binding protein-1: Molecular cloning, tissue distribution and gene expression level in response to nutritional regulation in mud crab, Scylla paramamosain.
Biochem Biophys Res Commun. 2018 Oct 03;:
Authors: Hao M, Lin Z, Rong H, Zhu D, Wen X
Abstract
In the present study, SREBP-1 cDNA was cloned from the hepatopancreas of mud crab (Scylla paramamosain) and characterized by performing rapid-amplification of cDNA ends. The 3361bp long full-length cDNA encodes a polypeptide with 1039 amino acids. Tissue distribution analysis revealed that SREBP-1 transcripts were widely distributed in various organs, with higher mRNA levels in the eyestalk and cranial ganglia. Further, expression level of SREBP-1 mRNA were up-regulated in proportion to the replacement of dietary fish oil (FO) with soybean oil (SO). These results may contribute to better understanding of the long-chain polyunsaturated fatty acids (LC-PUFA) biosynthetic pathway and regulation mechanism in mud crab.
PMID: 30292406 [PubMed - as supplied by publisher]

Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Hao M, Lin Z, Rong H, Zhu D, Wen X Tags: Biochem Biophys Res Commun Source Type: research