The comparison genomics analysis with glioblastoma multiforme (GBM) cells under 3D and 2D cell culture conditions

Publication date: 1 December 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 172Author(s): Liang Ma, Bin Zhang, Changchun Zhou, Yuting Li, Binjie Li, Mengfei Yu, Yichen Luo, Lei Gao, Duo Zhang, Qian Xue, Qingchong Qiu, Biaoyang Lin, Jun Zou, Huayong YangAbstractGBM, the most common and aggressive malignant primary brain tumors which needs new research approach to reveal the underline molecular mechanism of tumor progression. The 3D in vitro tumor model can be a simple and effective way to study tumor characteristics with ability to replicate of the tumor milieu. In the current study, we adopted the DNA microarray to analyze the gene expression of GBM tumor cells cultured under 2D cell culture flasks and 3D PLA porous scaffolds for 4,7 and 14 days. For 14 day old cultures, 8117 and 3060 genes expression were upregulated and downregulated respectively. Further KEGG pathway analysis revealed, the upregulated genes were mainly enriched/involved in PPAR and PI3K-Akt signaling pathways whereas the downregulated genes were mainly contributed in metabolism, ECM related and TGF-beta pathways. Thus, our approach of establishing 3D in vitro tumor model provides realistic results and proves itself a powerful tool for understanding the inner nature of GBM and can be considered as potential platform for drug screening.Graphical abstractThe comparison study of gene expression of GBM under 2D and 3D cell culture platforms for 4,7,14 days with DNA microarray.
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research