Poliovirus detection and genetic characteristic from sewage in Heilongjiang province from 2013 to 2016.

In this study, we collected 139 sewage samples from eight regions in Heilongjiang Province. Poliovirus was identified from 72 samples and the positive rate was 51%. 263 strains of poliovirus were isolated, including 22 type1, 104 type2 and 137 type3 polioviruses. As results of intratypic differentiation (ITD), using real time PCR and nucleotide sequencing, three type 1 pre-VDPV, one type 2 VDPV and two type 3 pre-VDPV strains were isolated, respectively. Interestingly, one type 1 strain with 5 nucleotide deletion and one type 3 recombinant on VP1 were isolated. By monitoring the poliovirus in the environment continually, we could recognize the VDPV or wild poliovirus with the high neurovirulence from large-scale circulation and set up warning system to avoid morbidity and transmission of the virus. PMID: 30068885 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research