(RS)-glucoraphanin purified from Tuscan black kale and bioactivated with myrosinase enzyme protects against cerebral ischemia/reperfusion injury in rats.

This study was aimed to examine the possible neuroprotective role of (RS)-glucoraphanin, bioactivated with myrosinase enzyme (bioactive RS-GRA), in an experimental rat model of brain ischemia/reperfusion injury (I/R). RS-GRA is a thiosaccharidic compound found in Brassicaceae, notably in Tuscan black kale (Brassica oleracea L. var. acephala sabellica). The mechanism underlying the inhibitory effects of bioactive RS-GRA on inflammatory and apoptotic responses, induced by carotid artery occlusion in rats, was carefully examined. Cerebral I/R was induced by clamping of carotid artery for 1h, followed by 40min of reperfusion through the release of clamp. Our results clearly have shown that administration of bioactive RS-GRA (10mg/Kg, i.p.) 15min after ischemia, significantly reduces proinflammatory parameters, such as inducible nitric oxide synthase expression (iNOS), intercellular adhesion molecule 1 (ICAM-1), nuclear factor (NF)-kB traslocation as well as the triggering of the apoptotic pathway (TUNEL and Caspase 3 expression). Taken together our data have shown that bioactive RS-GRA possesses beneficial neuroprotective effects in the counteracting the brain damage associated to I/R. Therefore, bioactive RS-GRA, could be an useful treatment in the cerebral ischemic stroke. PMID: 25281776 [PubMed - as supplied by publisher]
Source: Fitoterapia - Category: Biochemistry Authors: Tags: Fitoterapia Source Type: research