Neuroprotection against 6-OHDA toxicity in PC12 cells and mice through the Nrf2 pathway by a sesquiterpenoid from Tussilago farfara

Publication date: September 2018Source: Redox Biology, Volume 18Author(s): Joohee Lee, Kwangho Song, Eugene Huh, Myung Sook Oh, Yeong Shik KimAbstractOxidative stress plays a key role in neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. Therefore, the nuclear factor-E2-related factor 2 (Nrf2), a key regulator of the antioxidative response, is considered to be important as a therapeutic target for neurodegenerative diseases. We investigated the underlying mechanism of Nrf2-mediated neuroprotective effects against oxidative stress in the PC12 cell line by 7β-(3-ethyl-cis-crotonoyloxy)-1α-(2-methylbutyryloxy)-3,14-dehydro-Z-notonipetranone (ECN), one of the sesquiterpenoids in Farfarae Flos. Pretreatment of PC12 cells with ECN had a protective effect against hydrogen peroxide (H2O2)- or 6-hydroxydopamine (6-OHDA)-induced cytotoxicity. ECN upregulated the ARE-luciferase activity and induced the mRNA expression of Nrf2 and antioxidant enzyme heme oxygenase-1 (HO-1). Knockdown of Nrf2 by small, interfering RNA (siRNA) abrogated the upregulation of HO-1, indicating that ECN had induced HO-1 via the Nrf2 pathway. Pretreatment with the thiol reducing agents, N-acetylcysteine (NAC) or dithiothreitol (DTT), attenuated Nrf2 activation and HO-1 expression. However, the non-thiol reducing antioxidant, Trolox, failed to inhibit HO-1 induction by ECN. These results suggest that ECN may directly interact with Kelch-like ECH-associated protein 1 (Keap1) and mo...
Source: Redox Biology - Category: Biology Source Type: research

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ConclusionsThe most important variables associated with depression were poor perceived physical health, presence of difficulties in ADL, and female gender.ResumenIntroducciónLa depresión es un trastorno psiquiátrico incapacitante y frecuente en la edad adulta asociado a mayor mortalidad y discapacidad funcional.ObjetivosDeterminar la asociación de las variables clínicas y sociodemográficas con la depresión, en una muestra de personas mayores de 50 años residentes en España, y comparar la prevalencia de depresión con los demás países del est...
Source: Revista de Psiquiatria y Salud Mental - Category: Psychiatry Source Type: research
A pyrene-inhibitor fluorescent probe with large Stokes shift for the staining of Aβ1-42, α-synuclein, and amylin amyloid fibrils as well as amyloid-containing Staphylococcus aureus biofilms. Anal Bioanal Chem. 2018 Nov 09;: Authors: Mahía A, Conde-Giménez M, Salillas S, Pallarés I, Galano-Frutos JJ, Lasa Í, Ventura S, Díaz-de-Villegas MD, Gálvez JA, Sancho J Abstract Amyloid fibrils formed by a variety of peptides are biological markers of different human diseases, such as Alzheimer's disease, Parkinson's disease, and type II diabetes, and are struc...
Source: Analytical and Bioanalytical Chemistry - Category: Chemistry Authors: Tags: Anal Bioanal Chem Source Type: research
Authors: Lee W, Koh S, Hwang S, Kim SH Abstract Parkinson disease (PD) is the second most prevalent neurodegenerative disorder after Alzheimer disease. The loss of specific brain area, the substantia nigra pars compacta is known as a major etiology, however it is not fully understood how this neurodegeneration is initiated and what precisely causes this disease. As one aspect of pathophysiology for PD, synaptic dysfunction (synaptopathy) is thought to be an earlier appearance for neurodegeneration. In addition, some of the familial factors cumulatively exhibit that these factors such as α-synuclein, leucine-r...
Source: International Neurourology Journal - Category: Urology & Nephrology Tags: Int Neurourol J Source Type: research
Abstract Neurodegenerative diseases (NDs), including Alzheimer's disease (AD) and Parkinson's disease (PD), threaten the health of an ever-growing number of older people worldwide; so far, there are no effective cures. Significant efforts have been devoted to developing new drugs for NDs in recent years, and some small molecules have been shown to be promising in preclinical studies. However, the major challenge for brain-targeting drugs is how to efficiently deliver the drugs across the blood-brain barrier (BBB) to desired targets. To address this issue, liposomal delivery systems have proved to be ideal carriers...
Source: Drug Discovery Today - Category: Drugs & Pharmacology Authors: Tags: Drug Discov Today Source Type: research
New research reveals what gives coffee its protective properties for brain health, and why it can help keep Alzheimer's and Parkinson's disease at bay.
Source: Health News from Medical News Today - Category: Consumer Health News Tags: Nutrition / Diet Source Type: news
New research reveals what gives coffee its protective properties for brain health, and why it can help keep Alzheimer's and Parkinson's disease at bay.
Source: Parkinson's Disease News From Medical News Today - Category: Neurology Tags: Nutrition / Diet Source Type: news
Publication date: Available online 5 November 2018Source: MitochondrionAuthor(s): Komal Panchal, Anand Krishna TiwariAbstractNeurodegenerative diseases (NDs) are the group of disorder that includes brain, peripheral nerves, spinal cord and results in sensory and motor neuron dysfunction. Several studies have shown that mitochondrial dynamics and their axonal transport play a central role in most common NDs such as Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD) and Amyotrophic Lateral Sclerosis (ALS) etc. In normal physiological condition, there is a balance between mitochondrial fission and f...
Source: Mitochondrion - Category: Biochemistry Source Type: research
(University Health Network) A new study out of the Krembil Brain Institute, part of the Krembil Research Institute, suggests drinking coffee may protect you against developing both Alzheimer's and Parkinson's disease.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
(Rutgers University) Rutgers scientists have created a tiny, biodegradable scaffold to transplant stem cells and deliver drugs, which may help treat Alzheimer's and Parkinson's diseases, aging brain degeneration, spinal cord injuries and traumatic brain injuries. Stem cell transplantation, which shows promise as a treatment for central nervous system diseases, has been hampered by low cell survival rates, incomplete differentiation of cells and limited growth of neural connections.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
(Penn State) A new gene therapy can turn certain brain glial cells into functioning neurons, which in turn could help repair the brain after a stroke or during neurological disorders like Alzheimer's or Parkinson's diseases.
Source: EurekAlert! - Biology - Category: Biology Source Type: news
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