Joint toxicity on hepatic detoxication enzymes in goldfish (Carassius auratus) exposed to binary mixtures of lead and paraquat

Publication date: September 2018Source: Environmental Toxicology and Pharmacology, Volume 62Author(s): Xiaoming Xu, Zhaojie Cui, Shanshan WangAbstractCompared to single exposure, chemical mixtures might induce joint toxicity including additive, synergistic and antagonistic effects on both organisms and environment. Owing to the specific toxicity of oxidative stress and binding to proteins, lead (Pb) is generally recognized a non-essential and threatening heavy metal to animals and human. Paraquat (PQ) is a widely used herbicide in agriculture and can trigger oxidative stress as well as Pb. Little information was available about joint effects of the two chemicals on toxicological responses in organisms, especially in fish. In our present study, goldfish (Carassius auratus) were randomly exposed to single and combined experiments with different concentrations of Pb and PQ for 28 days. Activities of four enzyme biomarkers in liver, ethoxyresorufin-O-deethylase (EROD), 7-benzyloxy-4-trifluoromethyl-coumarin-O-debenzyloxylase (BFCOD), glutathione-S-transferase (GST) and UDP-glucuronosyltransferase (UGT) were evaluated in each experimental group on day 14 and 28. The results showed four enzyme levels were markedly reduced with the increase of concentrations in mixtures and prolonged exposure. The inhibitory EROD and BFCOD activities were not significantly changed in goldfish following PQ-treated groups with or without 0.5 mg/L Pb, which indicated PQ has more inhibitory toxicity...
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research