Methacholine induces extracellular matrix production by human airway smooth muscle cells through β-catenin signaling

This study attempted to investigate the role of methacholine in promoting ECM production by human ASMCs (HASMCs) and the underlying mechanism. We found that methacholine induced the expression of collagen I protein and multiple ECM genes. β-catenin signaling was activated in this process upon GSK3β phosphorylation, leading to upregulation of total and active β-catenin. Silencing β-catenin by specific small interfering RNA (siRNA) or with the β-catenin inhibitor, PKF115-584, decreased collagen I expression. Conversely, overexpression of active β-catenin by adenoviruses carrying the S33Y-β-catenin mutant increased the methacholine-induced collagen I expression. Furthermore, methacholine induced TGF-β expression in HASMCs, while pan-TGF-β-neutralizing antibody only partially decreased collagen I expression. These findings suggest that methacholine induced ECM production through β-catenin signaling and partially through TGF-β.
Source: Respiratory Physiology and Neurobiology - Category: Respiratory Medicine Source Type: research

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ConclusionThere is a clear correlation between tubulin acetylation and glucose uptake level.
Source: Archives of Cardiovascular Diseases Supplements - Category: Cardiology Source Type: research
Abstract An imprinting technique has been developed to generate synthetic polymer beads suitable for selectively binding a supramolecular target. The viral hexon protein, which is the most abundant and accessible surface protein component of the human Adenovirus type 5 (hAdV5) icosahedral capsid, was applied as the template molecule to generate functional polymer beads entailing selectivity for the entire virus. Individual and competitive rebinding studies using two different viruses (i.e. hAdV5 and Minute Virus of Mice - MVM) revealed exquisite selectivity of the imprinted beads for the target hAdV5. Additionally...
Source: Appl Human Sci - Category: Physiology Authors: Tags: Mater Sci Eng C Mater Biol Appl Source Type: research
LetterCheston B. Cunha,Infection Control&Hospital Epidemiology,Volume 37 Issue 09, pp 1127-1129Abstract
Source: Infection Control and Hospital Epidemiology - Category: Epidemiology Source Type: research
Centronuclear myopathies (CNMs) are severe diseases characterized by muscle weakness and myofiber atrophy. Currently, there are no approved treatments for these disorders. Mutations in the phosphoinositide 3-phosphatase myotubularin (MTM1) are responsible for X-linked CNM (XLCNM), also called myotubular myopathy, whereas mutations in the membrane remodeling Bin/amphiphysin/Rvs protein amphiphysin 2 [bridging integrator 1 (BIN1)] are responsible for an autosomal form of the disease. Here, we investigated the functional relationship between MTM1 and BIN1 in healthy skeletal muscle and in the physiopathology of CNM. Genetic o...
Source: Science Translational Medicine - Category: Biomedical Science Authors: Tags: Research Articles Source Type: research
Abstract An excessive inflammatory response in terminal airways, alveoli, and the lung interstitium eventually leads to pulmonary hypertension and chronic obstructive pulmonary disease. Proinflammatory cytokine interleukin-17A (IL-17A) has been implicated in the pathogenesis of pulmonary inflammatory diseases. MLN4924, an inhibitor of NEDD8-activating enzyme (NAE), is associated with the treatment of various types of cancers, but its role in the IL-17A-mediated inflammatory response has not been identified. Here, we report that MLN4924 can markedly reduce the expression of proinflammatory cytokines and chemokines ...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - Category: Cytology Authors: Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research
Abstract Recently, mitochondria have been shown to have a vital role in the innate immune response to viral infection. In response, viruses such as adenovirus, have developed mechanisms to alter mitochondrial function through direct or indirect interaction with the mitochondria. The interaction of human recombinant adenoviral vectors directly with human mitochondria has not previously been shown. We demonstrate that human recombinant adenoviral vectors co-localize to mitochondria. We show that the adenoviral vectors are present within the membranes of the mitochondria and that they cause ultrastructural changes to...
Source: Acta Virologica - Category: Infectious Diseases Authors: Tags: Acta Virol Source Type: research
In conclusion, we demonstrated that the VP3 expression mediated by rAAAV in ducklings could induce protective immunity against DHAV challenge, and this could be a candidate vaccine for the control of duck viral hepatitis. Keywords: avian adeno-associated virus; duck hepatitis A virus; VP3 gene; immunogenicity. PMID: 30879313 [PubMed - in process]
Source: Acta Virologica - Category: Infectious Diseases Authors: Tags: Acta Virol Source Type: research
This article aims to provide readers with detailed technical information for rapid production and validation of high-quality viral particles in a laboratory setting while highlighting advantages and limitations of each viral type. © 2019 by John Wiley &Sons, Inc. PMID: 30883041 [PubMed - as supplied by publisher]
Source: Current Protocols in Neuroscience - Category: Neuroscience Authors: Tags: Curr Protoc Neurosci Source Type: research
CONCLUSIONS: Our findings reveal a novel endogenous cardioprotective mechanism that long-term exercise-derived circulating exosomes protect the heart against MI/R injury via exosomal miR-342-5p. PMID: 30879399 [PubMed - as supplied by publisher]
Source: Circulation Research - Category: Cardiology Authors: Tags: Circ Res Source Type: research
Publication date: Available online 15 March 2019Source: The Lancet HIVAuthor(s): Frances H Priddy, David J M Lewis, Huub C Gelderblom, Hana Hassanin, Claire Streatfield, Celia LaBranche, Jonathan Hare, Josephine H Cox, Len Dally, Daryl Bendel, David Montefiori, Eddy Sayeed, Jim Ackland, Jill Gilmour, Bruce C Schnepp, J Fraser Wright, Philip JohnsonSummaryBackgroundA preventive vaccine for HIV is a crucial public health need; adeno-associated virus (AAV)-mediated antibody gene delivery could be an alternative to immunisation to induce sustained expression of neutralising antibodies to prevent HIV. We assessed safety and tol...
Source: The Lancet HIV - Category: Infectious Diseases Source Type: research
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