Investigation of immune cell markers in feline oral squamous cell carcinoma

Publication date: August 2018Source: Veterinary Immunology and Immunopathology, Volume 202Author(s): Ellen E. Sparger, Brian G. Murphy, Farina Mustaffa Kamal, Boaz Arzi, Diane Naydan, Chrisoula T. Skouritakis, Darren P. Cox, Katherine SkorupskiAbstractSquamous cell carcinoma is the most common oral cancer in the cat and presents as a locally aggressive lesion for which an effective therapeutic protocol remains elusive. Feline oral squamous cell carcinoma (OSCC) shares many clinical characteristics with human head and neck squamous cell carcinoma (HNSCC). Accordingly, present studies were conducted to determine similarities for immune markers shared by feline OSCC and human HNSCC. Biopsies harvested from a feline patient cohort-1 (n = 12) were analyzed for lymphoid cell infiltrates by immunohistochemistry (IHC). Results revealed unique patterns of T cell infiltration involving both neoplastic epithelium and stroma that were detected in most patient tumor biopsies (92%) examined by IHC staining for CD3. Intratumoral B cell infiltrates were detected within tumor stroma only, based on IHC staining for CD79a and CD20 for all patients within the same cohort-1. Infiltration of tumors by a regulatory CD4 T cell subset (Tregs) defined by expression of the forkhead transcription factor FoxP3, was also detected in biopsies from 57% of patients and involved infiltration of neoplastic epithelium and stroma. Patient biopsies were also examined for expression of immunomodulator cyclooxy...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research