Tissue-associated self-antigens containing exosomes: Role in allograft rejection.

Tissue-associated self-antigens containing exosomes: Role in allograft rejection. Hum Immunol. 2018 Jun 14;: Authors: Sharma M, Ravichandran R, Bansal S, Bremner RM, Smith MA, Mohanakumar T Abstract Exosomes are extracellular vesicles that express self-antigens (SAgs) and donor human leukocyte antigens. Tissue-specific exosomes can be detected in the circulation following lung, heart, kidney and islet cell transplantations. We collected serum samples from patients who had undergone lung (n=30), heart (n=8), or kidney (n=15) transplantations to isolate circulating exosomes. Exosome purity was analyzed by Western blot, using CD9 exosome-specific markers. Tissue-associated lung SAgs, collagen V (Col-V) and K-alpha 1 tubulin (Kα1T), heart SAgs, myosin and vimentin, and kidney SAgs, fibronectin and collagen IV (Col-IV), were identified using western blot. Lung transplant recipients diagnosed with bronchiolitis obliterans syndrome had exosomes with higher expression of Col-V (4.2 fold) and Kα1T (37.1fold) than stable. Exosomes isolated from heart transplant recipients diagnosed with coronary artery vasculopathy had a 3.9 fold increase in myosin and a 4.7fold increase in vimentin compared with stable. Further, Kidney transplant recipients diagnosed with transplant glomerulopathy had circulating exosomes with a 2 fold increased expression of fibronectin and 2.5 fold increase in Col-IV compared with stable. We conclude that circulating exos...
Source: Human Immunology - Category: Allergy & Immunology Authors: Tags: Hum Immunol Source Type: research