Untargeted metabolomics reveals predominant alterations in primary metabolites of broccoli sprouts in response to pre-harvest selenium treatment

In this study, an untargeted metabolomics approach was developed to investigate the effect of 5 days of treatment with 100 μmol/L selenate on the metabolome of broccoli sprouts. Results showed that the total sulfur content was slightly lower and the total selenium content was significantly higher in the selenate-treated sprouts. The multivariate statistical analyses showed that serine, d-erythronolactone, melezitose and tyrosine were obviously up-regulated, but d-glyceric acid, succinic acid and citric acid were down-regulated after selenate treatment. Moreover, metabolite pathway analyses were used to support the identification of subtle but significant changes among groups of related metabolites that cannot be observed with conventional approaches. Selenate treatment influenced the metabolism of β-alanine and glutathione, as well as the biosynthesis of plant metabolite related to the precursors of glucosinolate. These results could explain why the total glucosinolate decreased after selenate treatment. Graphical abstract
Source: Food Research International - Category: Food Science Source Type: research