Molecular basis of the counteraction by calcium channel blockers of Cyclosporine nephrotoxicity.

In this study, we tested the hypothesis that administration of calcium channel blockers such as verapamil or nifedipine ameliorate renal CSA-induced renal dysfunction. Furthermore, our study investigates the roles of inflammatory, oxidative, and fibrotic pathways in CSA-induced renal dysfunction. Six groups of male rats (n=6/group) were used and received one of the following treatments for 7 consecutive days: vehicle (cremophor EL, i.p.), CSA (25 mg.kg-1.day-1, i.p.), verapamil (2 mg.kg-1.day-1, i.p.), nifedipine (3 mg.kg-1.day-1, i.p.), CSA in the presence or absence of either verapamil, or nifedipine. Biochemical and histomorphometric analyses showed that rats treated with CSA exhibited clear signs of nephrotoxicity that included: (i) proteinuria and elevations in serum creatinine and blood urea nitrogen, (ii) Mesangial expansion (iii) increases in glomerular and tubular type IV collagen expression, and (iv) increases in the glomerulosclerosis and tubulointerstitial fibrosis indices. While the single administration of nifedipine or verapamil had no significant effect on renal pathology, or its biochemical and physiological function, the concurrent use of either calcium channel blockers significantly and equipotently ameliorated the biochemical, morphological and functional derangements caused by CSA. More importantly, we report that the oxidative (ROS production, NADPH-oxidase activity and DUOX1/2 levels), fibrotic (TGF-β1 expression) and inflammatory (NF-κB expr...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research

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ConclusionsBaseline diabetes may be independently associated with failure to achieve complete remission, but not with IMN relapse and renal function decline in IMN patients.
Source: International Urology and Nephrology - Category: Urology & Nephrology Source Type: research
Authors: Chen FF, Tang HY, Yu F, Que CL, Zhou FD, Wang SX, Wang GF, Zhao MH Abstract A 43-year-old Chinese man with a silicosis history was admitted to our hospital due to bilateral lower extremity edema for 1 year, exacerbating with hematuria for 2 months. He started working as a coal miner 30 years ago, and was diagnosed as silicosis 3 months ago. Lab tests revealed hematuria 3+, proteinuria 3+, and a serum creatinine value 2.47 mg/dl on routine check. He was diagnosed with focal proliferative IgA nephropathy (IgAN) and acute tubulo-interstitial nephritis by renal biopsy. He was...
Source: Renal Failure - Category: Urology & Nephrology Tags: Ren Fail Source Type: research
Conclusion: the therapeutic effect of small molecule targeting drug apatinib on gastric cancer is better than that of other drugs, whether in therapeutic effect, drug resistance, adverse reactions or infection control. This study has important reference significance for the follow-up treatment of apatinib and cancer.
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research
We report our experience with pediatric NS in the last 18 years.
Source: Journal of Pediatric Urology - Category: Urology & Nephrology Authors: Source Type: research
ConclusionsUPCR showed strong correlation and consistency with 24HUPr for evaluating levels of proteinuria in children. UPCR
Source: Pediatric Nephrology - Category: Urology & Nephrology Source Type: research
The objective of this study was to determine the association of Computed tomography quantified visceral (VAT) and subcutaneous adipose tissue (SAT) with estimated glomerular filtration rate (eGFR) using CKD-EPI formula, diabetes mellitus, body mass index (BMI), proteinuria, lipid profile, and hypertension. Increased adipose tissue is the primary phenotypic characteristic of obesity. The amount and distribution of adipose tissue is associated with many adverse consequences, as hypertension, type 2 diabetes, cardiovascular disease etc. Abdominal CT with semi-automated software can quantify adipose tissue and predict the risk...
Source: Journal of the Association of Physicians of India - Category: General Medicine Tags: J Assoc Physicians India Source Type: research
ConclusionTaken together, this work suggests a previously under-appreciated role for OCRL in glomerular function and highlights the importance of investigating tubular function in patients with persistent proteinuria.
Source: Pediatric Nephrology - Category: Urology & Nephrology Source Type: research
We examined a patient aged 31 who had a sudden burning paraesthesia, pain and numbness in the lower legs together with an increased temperature of 39°C. Clinical examination showed asymmetrical sensory polyneuropathy more clearly seen in the lower legs and the left wrist, with high ESR (up to 44 mm/h), leukocytosis, slight anemia and proteinuria. CSF was normal. After three weeks the temperature suddenly increased again up to 39°C and severe flaccid distal tetraparesis was seen more clearly with foot drop in the left lower leg and dense oedema in the left wrist, purple cyanosis and haemorrhagic foci appeared on the...
Source: Acta Myologica - Category: Neurology Tags: Acta Myol Source Type: research
This study aimed to analyse maternal and foetal condition and pregnancy outcomes of PE patients with different IOS, and to explore the disease progression and characteristics of maternal and foetal outcomes for different IOS, so as to provide the basis for future maternal and foetal monitoring of PE patients.Impact statementWhat is already known on this subject? In 2013, the American College of Obstetricians and Gynecologists revised their definition of PE, sparking a heated debate. Subsequently in 2015, China updated its guidelines to define PE as hypertensive pregnancy accompanied by involvement of any other organ or org...
Source: Journal of Obstetrics and Gynaecology - Category: OBGYN Tags: J Obstet Gynaecol Source Type: research
ConclusionFew case reports have described histological findings of proteinase 3-antineutrophil cytoplasmic antibody-positive renal injury complicated with infective endocarditis. We believe that an accumulation of histological findings and treatments is mandatory for establishment of optimal management for proteinase 3-antineutrophil cytoplasmic antibody-positive renal injury complicated with infective endocarditis.
Source: Journal of Medical Case Reports - Category: General Medicine Source Type: research
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