GSE107962 MicroRNAs into body fluids from ten organs of mice exposed to cigarette smoke

Contributors : Alberto Izzotti ; Mariagrazia Longobardi ; Sebastiano La Maestra ; Rosanna T Micale ; Alessandra Pulliero ; Anna Camoirano ; Marta Geretto ; Francesco D'Agostini ; Rumen Balansky ; Mark Miller ; Vernon Steele ; Silvio De FloraSeries Type : Non-coding RNA profiling by arrayOrganism : Homo sapiens ; Mus musculus ; Rattus norvegicusThe purpose of the present study was to validate the application of this epigenetic biomarker by using less invasive collection procedures.Using microarray analyses, we measured 1135 microRNAs in 10 organs and 3 body fluids of mice that were either unexposed or exposed to mainstream cigarette smoke for up to 8 weeks. The results obtained with selected miRNAs were validated by qPCRThe lung was the main target effected by smoke (190 dysregulated miRNAs), followed by skeletal muscle (180), liver (138), blood serum (109), kidney (96), spleen (89), stomach (36), heart (33), bronchoalveolar lavage fluid (32), urine (27), urinary bladder (12), colon (5), and brain (0). Skeletal muscle, kidney, and lung were the most important sources of smoke-altered microRNAs in blood serum, urine, and bronchoalveolar lavage fluid, respectively
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by array Homo sapiens Mus musculus Rattus norvegicus Source Type: research