Cultural and immunohistochemical evaluation of bacterial agents in caprine pneumonia in Nigeria

This study evaluates the prevalence of important bacterial pathogens associated with caprine pneumonia in Nigeria using cultural and immunohistochemical techniques. One hundred and fifty goat lungs were randomly examined from macroscopic, cultural isolation and microscopic changes using standard techniques. The common bacteria identified were formalin inactivated for bacterine and polyclonal antibody production. Immunohistochemical staining was performed against the bacteria antigens using the avidin-biotin peroxidase complex technique. The data were presented in frequencies and percentages, and compared using non-parametric statistics atα = 0.05. The pathology in the caprine lungs included broncho-interstitial pneumonia (41), interstitial pneumonia (27), and bronchopneumonia (72). Ten of the lungs were normal. Bacterial isolation yieldedMannheimia haemolytica (40%),Pasteurella multocida (20%),Escherichia coli (13%),Klebsiella pneumoniae (10%),Streptococcus sp. (8%),Baccillus sp. (5%),and Pseudomonas sp. (3%).Mannheimia haemolytica (Mh) andPasturella multocida (Pm) were the most common isolated bacteria. The bacterial antigens were detected on the surface of respiratory bronchiolar, and alveolar epithelia, within inflammatory exudate and in cytoplasm of alveolar macrophages. One hundred twenty (80%) of the caprine lungs were positive for theMh andPm antigens; 47 forMh, 59 forPm, 14 for both, and 30 negative for either antigens. Significant differences were observed in the...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research