Multicenter Evaluation of the Accelerate PhenoTest BC Kit for Rapid Identification and Phenotypic Antimicrobial Susceptibility Testing Using Morphokinetic Cellular Analysis Bacteriology

We describe results from a multicenter study evaluating the Accelerate Pheno system, a first of its kind diagnostic system that rapidly identifies common bloodstream pathogens from positive blood cultures within 90 min and determines bacterial phenotypic antimicrobial susceptibility testing (AST) results within ~7 h. A combination of fresh clinical and seeded blood cultures were tested, and results from the Accelerate Pheno system were compared to Vitek 2 results for identification (ID) and broth microdilution or disk diffusion for AST. The Accelerate Pheno system accurately identified 14 common bacterial pathogens and two Candida spp. with sensitivities ranging from 94.6 to 100%. Of fresh positive blood cultures, 89% received a monomicrobial call with a positive predictive value of 97.3%. Six common Gram-positive cocci were evaluated for ID. Five were tested against eight antibiotics, two resistance phenotypes (methicillin-resistant Staphylococcus aureus and Staphylococcus spp. [MRSA/MRS]), and inducible clindamycin resistance (MLSb). From the 4,142 AST results, the overall essential agreement (EA) and categorical agreement (CA) were 97.6% and 97.9%, respectively. Overall very major error (VME), major error (ME), and minor error (mE) rates were 1.0%, 0.7%, and 1.3%, respectively. Eight species of Gram-negative rods were evaluated against 15 antibiotics. From the 6,331 AST results, overall EA and CA were 95.4% and 94.3%, respectively. Overall VME, ME, and mE rates were 0.5%, ...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Bacteriology Source Type: research

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Publication date: Available online 9 December 2019Source: PolymerAuthor(s): O.I. Timaeva, N.A. Arkharova, A.S. Orekhov, V.V. Klechkovskaya, S.P. Mulakov, G.M. Kuz'micheva, I.I. PashkinAbstractComposite hydrogels in the poly-N-vinylpyrrolidone – RE(NO3)3 × xH2O system with RE = La, Gd, Yb were prepared in various forms (native, native dried in the air, dried in air and ground into powder). They were studied by methods of wide- and small-angle X-ray scattering, differential scanning calorimetry, scanning electron microscopy, and electron microprobe analysis. The composition of native PVP and PVP with powd...
Source: Polymer - Category: Chemistry Source Type: research
Over the past few decades, antimicrobial resistance has emerged as an important threat to public health due to the global dissemination of multidrug-resistant strains from several bacterial species. This worrisome trend, in addition to the paucity of new antibiotics with novel mechanisms of action in the development pipeline, warrants the development of non-antimicrobial approaches to combating infection caused by these isolates. Monoclonal antibodies (mAbs) have emerged as highly effective molecules for the treatment of multiple diseases. However, in spite of the fact that antibodies play an important role in protective i...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
The Centers for Disease Control and Prevention (CDC) has recently identified medical devices used in the ICU as a major source of antibiotic-resistant infections. N8 Medical’s CeraShield technology may help reduce such infections by preventing fouling and formation of bacterial and fungal biofilms on device surfaces. FDA has designated the company’s CeraShield Endotracheal Tube (ETT) as a “breakthrough” device, pursuant to the 21st Century Cures Act based on its potential life-saving properties. And N8 reported that ...
Source: MDDI - Category: Medical Devices Authors: Tags: Materials Source Type: news
This study provides a promising strategy to develop hybrid nanocomposite with enhanced antibacterial efficacy and also opens a new way for the establishment of efficient therapeutic systems with reduced administration frequency as substitute of antibiotics to treat otitis media.Graphical abstract
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
Publication date: Available online 9 December 2019Source: International Journal of PharmaceuticsAuthor(s): Mohammed Salih, Calvin A. Omolo, Nikhil Agrawal, Pavan Walvekar, Ayman Y. Waddad, Chunderika Mocktar, Charlotte Ramdhin, Thirumala GovenderAbstractThe global threat of antimicrobial resistant strains calls for innovative strategies to utilize nano drug delivery systems to enhance the delivery of antibiotics, thus reducing the development of resistance. Supramolecular amphiphiles that can self-assemble into nanostructures are one such nano delivery system, that are showing potential for effective drug delivery. The aim...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
MONDAY, Dec. 9, 2019 -- The risk of new superbugs that pose a threat to human health and food crops is much higher than previously thought, new genetic research shows. There's been an uptick in the number of laboratory studies showing how just one...
Source: Drugs.com - Daily MedNews - Category: General Medicine Source Type: news
Publication date: Available online 9 December 2019Source: The Journal of Allergy and Clinical Immunology: In PracticeAuthor(s): Emily H. Liang, Lie H. Chen, Eric MacyAbstractBackgroundLimited population-based data on penicillin-, carbapenem-, monobactam-, and clindamycin-associated reported adverse reactions exists.ObjectiveCollect data on penicillin, carbapenem, monobactam, and clindamycin usage and associated adverse reactions.MethodsData from 1-1-2009 to 12-31-2017 in Kaiser Permanente Southern California was collected.ResultsThere were 6,144,422 unique individuals, mean age 33.6 ± 21.1 years, 52.2% females, with...
Source: The Journal of Allergy and Clinical Immunology: In Practice - Category: Allergy & Immunology Source Type: research
(University of Texas Health Science Center at Houston) Researchers at The University of Texas Health Science Center at Houston (UTHealth) have identified a protein that allows vancomycin-resistant enterococci, a deadly superbug, to defy antibiotic treatment and immune system attacks. Their discovery opens the door for future treatment options in the fight against antibiotic resistance.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
Source: Infection and Drug Resistance - Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
Source: The Journal of Thoracic and Cardiovascular Surgery - Category: Cardiovascular & Thoracic Surgery Authors: Source Type: research
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