O-031 Combinational therapy following elvo in a mouse model of stroke demonstrates new frontiers in neuroprotection: the mavaric trial

Stroke remains a leading cause of morbidity and mortality in the United States. Potential therapies have encountered significant barriers in attempts to translate bench to bedside research. Because of this, we have evaluated novel roles for FDA approved drugs, repurposed for treating stroke. Two such drugs, verapamil and magnesium, represent drug classes with a long history in neuroprotection trials with mixed results. Using an intra-arterial (IA) model developed in our lab, we selectively delivered our agents of interest to the stroke affected region following experimental stroke and successful recanalization. In addition, we studied the effect of the drugs on primary cortical neurons (PCN) exposed to oxygen glucose deprivation (OGD) in vitro. In vivo experiments used 16 week old C57/Bl6 male mice that underwent a tandem transient common carotid/middle cerebral artery occlusion for 60 min. Following successful recanalization, verapamil (1.76 mg/kg) and magnesium (176 mg/kg) were administered IA through the internal carotid artery using a previously determined flow rate and injection volume. Physiological measurements (heart rate and blood pressure) were monitored for potential deleterious effects during and ten minutes following IA drug administration. A neurological score was used to determine differences in functional outcome on post-stroke days (PSD) 1 and 7. On PSD 7, animals were euthanized and brains flash frozen for immunohistochemistry analysis of infarct volume, ma...
Source: Journal of NeuroInterventional Surgery - Category: Neurosurgery Authors: Tags: Oral Abstracts Source Type: research