miR ‐339‐5p downregulation contributes to Taxol resistance in small‐cell lung cancer by targeting α1,2‐fucosyltransferase 1

This study investigated the role of miR‐339‐5p involvement in lung carcinoma cell lines and chemoresistance to Taxol. We observed that miR‐339‐5p was significantly downregulated in Taxol‐A549 cells compared with A549 cells. In vitro studies further indicated that miR‐339‐5p could promote colony formation and attenuate apoptosis of lung carcinoma cell lines through targeting α1,2‐fucosyltransferase 1 and regulation of the downstream protein Lewis y. Furthermore, miR‐339‐5p was found to enhance the proliferation inhibition ability of Taxol in lung carcinoma cell lines as well as in the Taxol‐A549 subclone. An in vivo study indicated that both miR‐339‐5p and Taxol could attenuate the growth of lung carcinoma; moreover, miR‐339‐5p could synergistically promote this inhibitory function of Taxol. In summary, our results suggest a miR‐339‐5p molecular network that is involved in controlling lung carcinoma progression. © 2017 The Authors IUBMB Life published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology, 2017
Source: IUBMB Life - Category: Research Authors: Tags: Research Communication Source Type: research