Recombinant Bacillus subtilis spores expressing cholera toxin B subunit and Helicobacter pylori urease B confers protection against H. pylori in mice.

In this study, cholera toxin B subunit (CTB), a mucosal adjuvant, was used to enhance the immunogenicity of novel Bacillus subtilis spores vaccine that express CTB-UreB along with the B. subtilis spore coat protein CotC as a fusion protein. Oral administration of B. subtilis spores expressing CotC-UreB or CotC-CTB-UreB led to increased levels of UreB-specific IgG in serum and UreB-specific IgA in feces, as well as elevated levels of interleukin-10 and interferon-γ in splenocytes. In addition, oral administration of CotC-UreB or CotC-CTB-UreB spores induced significant reductions (80.0% and 90.5%, respectively) in stomach H. pylori bacterial load (1.11 ± 0.36 × 105 CFU and 0.53 ± 0.21 × 105 CFU, respectively) compared to that of the CotC control group (5.56 ± 1.64 × 105 CFU, p < 0.01). Moreover, CotC-CTB-UreB spores were significantly more effective at reducing the bacterial load than CotC-UreB spores (p < 0.05). These results indicate that CotC-CTB-UreB-expressing B. subtilis spores is a potential vaccine candidate for the control of H. pylori infection. PMID: 27930268 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research