The origin of the PB1 segment of swine influenza A virus subtype H1N2 determines viral pathogenicity in mice.

The origin of the PB1 segment of swine influenza A virus subtype H1N2 determines viral pathogenicity in mice.
Virus Res. 2014 Apr 12;
Authors: Metreveli G, Gao Q, Mena N, Schmolke M, Berg M, Albrecht RA, García-Sastre A
Abstract
Swine appear to be a key species in the generation of novel human influenza pandemics. Previous pandemic viruses are postulated to have evolved in swine by reassortment of avian, human, and swine influenza viruses. The human pandemic influenza viruses that emerged in 1957 and 1968 as well as swine viruses circulating since 1998 encode PB1 segments derived from avian influenza viruses. Here we investigate the possible role in viral replication and virulence of the PB1 gene segments present in two swine H1N2 influenza A viruses, A/swine/Sweden/1021/2009(H1N2) (sw 1021) and A/swine/Sweden/9706/2010(H1N2) (sw 9706), where the sw 1021 virus has shown to be more pathogenic in mice. By using reverse genetics, we swapped the PB1 genes of these two viruses. Similar to the sw 9706 virus, chimeric sw 1021 virus carrying the sw 9706 PB1 gene was not virulent in mice. In contrast, replacement of the PB1 gene of the sw 9706 virus by that from sw 1021 virus resulted in increased pathogenicity. Our study demonstrated that differences in virulence of swine influenza virus subtype H1N2 are attributed at least in part to the PB1 segment.
PMID: 24726997 [PubMed - as supplied by publisher]

Source: Virus Research - April 12, 2014 Category: Virology Authors: Metreveli G, Gao Q, Mena N, Schmolke M, Berg M, Albrecht RA, García-Sastre A Tags: Virus Res Source Type: research