Norepinephrine Inhibits Mesenchymal Stem Cell Chemotaxis Migration by Increasing Stromal Cell-derived Factor-1 Secretion by Vascular Endothelial Cells via NE/abrd3/JNK Pathway.

Norepinephrine Inhibits Mesenchymal Stem Cell Chemotaxis Migration by Increasing Stromal Cell-derived Factor-1 Secretion by Vascular Endothelial Cells via NE/abrd3/JNK Pathway. Exp Cell Res. 2016 Sep 17; Authors: Wu B, Wang L, Yang X, Mao M, Ye C, Liu P, Yang Z, Yang X, Lei D, Zhang C Abstract Mesenchymal stem cells (MSCs), which are physiologically maintained in vascular endothelial cell (VEC)-based niches, play a critical role in tissue regeneration. Our previous studies demonstrated that sympathetic denervation could promote MSC mobilization, thereby enhancing bone formation in distraction osteogenesis (DO), a self-tissue engineering for craniofacial and orthopeadic surgeries. However, the mechanisms on how sympathetic neurotransmitter norepinephrine (NE) regulates MSC migration are not well understood. Here we showed that deprivation of NE by transection of cervical sympathetic trunk (TCST) inhibited stromal cell-derived factor-1 (SDF-1) expression in the perivascular regions in rat mandibular DO. In vitro studies showed that NE treatment markedly upregulated p-JNK and therefore stimulated higher SDF-1 expression in VECs than control groups, and siRNA knockdown of the abrd3 gene abolished the NE-induced p-JNK activation. On the other hand, osteoblasts differentiated from MSCs showed an increase in SDF-1 secretion with lack of NE. Importantly, NE-treated VECs inhibited the MSC chemotaxis migration along the SDF-1 concentration gra...
Source: Experimental Cell Research - Category: Cytology Authors: Tags: Exp Cell Res Source Type: research