Neuroprotective effects of adenosine isolated from Cordyceps cicadae against oxidative and ER stress damages induced by glutamate in PC12 cells

In this study, the protective effects of adenosine from Cordyceps cicadae against glutamate-induce oxidative stress in PC12 cells was evaluated. The exposure of PC12 cells to glutamate (5mM) induced the formation of ROS, increased Ca2+ influx, endoplasmic reticulum (ER) stress and up regulated the expression of pro-apoptotic factor Bax. However, pretreatment with adenosine markedly increased cell viability, decreased the elevated levels of ROS and Ca2+ induced by glutamate. Furthermore adenosine increased the activities of GSH-Px and SOD, as well as retained mitochondria membrane potential (MMP), increased Bcl-2/Bax ratio, and reduced the expression of ERK, p38, and JNK. Overall, our results suggest that adenosine may be a promising potential therapeutic agent for the prevention and treatment of neurodegenerative disorders.
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research