Induction of endothelial phenotype from Warton Jelly-derived MSC and comparison of their vaso- and neuro-protective potential with primary WJ-MSC in CA1 hippocampal region ex vivo.

Induction of endothelial phenotype from Warton Jelly-derived MSC and comparison of their vaso- and neuro-protective potential with primary WJ-MSC in CA1 hippocampal region ex vivo. Cell Transplant. 2015 Dec 31; Authors: Obtulowicz P, Lech W, Strojek L, Sarnowska A, Domanska-Janik K Abstract Ischemic stroke results in violent impairment of tissue homeostasis leading to severe perturbation within the neurovascular unit (NVU) during recovery period. The aim of study was to assess the potential of mesenchymal stem cells (MSC) originating from Wharton jelly (WJ) to differentiate into functionally competent cells of endothelial lineage (WJ-EPC). Protective effect of either primary WJ-MSC or induced WJ-EPC was investigated and compared after oxygen-glucose deprivation (OGD) of hippocampal organotypic slices (OHC) in the indirect co-culture model. WJ-EPC, primed in EGM-2 media under 5% O2, acquired cobblestone endothelial-like morphology, formed capillary-like structures and actively took up DiI-Ac-LDL. The both cell types (WJ-MSC and WJ-EPC) were positive for CD73, CD90, CD105, VEGFR-2, VEGF, but only endothelial one expressed vWF and PECAM-1 markers at significant levels. In the presence of either WJ-MSC or WJ-EPC culture at compartment below of OGD-injured slices, substantially decreased cell death and vascular atrophy in hypoxia-sensitive CA1 region. This suggests paracrine mechanism of both WJ-MSC and WJEPC-dependent protection. Thus, f...
Source: Cell Transplantation - Category: Cytology Authors: Tags: Cell Transplant Source Type: research