Serum bacterial DNA detection in patients with cholangitis after Kasai procedure

Abstract BackgroundThe aim of this study was to investigate the detectability of bacterial DNA (bactDNA) and associated clinical factors in patients with cholangitis after the Kasai procedure through the identification of bactDNA in blood. MethodsA cross‐sectional study of 110 patients who had undergone the Kasai procedure was carried out. A total of 77 patients had cholangitis and 33 did not. Blood samples were obtained on admission or on follow up at the outpatient visit. bactDNA was analyzed using a polymerase chain reaction‐based method, DNA nucleotide sequencing, for identification of bacterial species. ResultsRate of bactDNA positivity was higher using the 16SrDNA method than the culture method (58.4% vs 14.3%, P 
Source: Pediatrics International - Category: Pediatrics Authors: Tags: Original Article Source Type: research

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Authors: Dong Y, Li G Abstract Nonalcoholic fatty liver disease (NAFLD) is one of the most common forms of chronic liver disease in developed countries and is associated with type 2 diabetes mellitus, obesity, hypertension, dyslipidemia, and metabolic syndrome. It is defined as steatosis in over 5% of hepatocytes. The disease spectrum of NAFLD ranges from simple fatty liver to nonalcoholic steatohepatitis, liver fibrosis, even hepatic cirrhosis. The disease affects various extra-hepatic systems such as the cardiovascular system and urinary system. Heart-related disease is identified as the leading cause of mor...
Source: Herz - Category: Cardiology Tags: Herz Source Type: research
ConclusionTo our knowledge, this is the first report on complete nucleotide sequences of type 2 IncC plasmids. These findings, which hypothesise the acquisition of KPC-2-encoding transposon Tn4401a by an IncC replicon, indicate the ongoing need for molecular surveillance studies of multidrug-resistant pathogens. In addition, they underline the increasing clinical importance of the IncC plasmid family.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
ConclusionOur results suggest that steatotic grafts should be used for recipients without severe liver failure to avoid ABS, and that endoscopic stenting of post-LT ABS leads to a high success rate, but is associated with a significant risk of recurrence.
Source: Clinics and Research in Hepatology and Gastroenterology - Category: Gastroenterology Source Type: research
Authors: Siwiec E, Szynkowska A, Milkiewicz M, Marlicz W, Stachowska E Abstract Conjugated dienes of linoleic acid (CLA) are constitutional and geometric isomers of linoleic acid that are commonly used as dietary supplements during body mass reduction. Their role in the reduction of lipid deposits in liver tissue is not unequivocal. CLA contain an equimolar mixture of two isomers of linoleic acid: trans-10,cis-12 CLA and cis-9,trans-11. Only one isomer - trans-10,cis-12 CLA exhibits fat-reducing properties, cis-9,trans-11 CLA does not. The main goal of this study was to determine if CLA isomers affect the activatio...
Source: Journal of Physiology and Pharmacology - Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research
Authors: Skladany L, Koller T, Molcan P, Vnencakova J, Zilincan M, Jancekova D, Kukla M Abstract In hospitalized patients with advanced chronic liver disease (ACLD), we aimed to evaluate the association between myostatin and muscle mass, its relation to inflammation and to assess the added prognostic value of myostatin for survival. In a prospective single-center cohort study, inclusion criteria were: consecutive hospitalization for ACLD and Child-Pugh score ≥ 7 points. Baseline parameters were myostatin, C-reactive protein (CRP), hand-grip strength (HGS), mid-arm muscle circumference (MAC), transversal psoas mu...
Source: Journal of Physiology and Pharmacology - Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research
Authors: DENTAL SUPPLEMENT, Oberti L, Gabrione F, Greco G, Severino M Abstract Osseointegrated dental implants showed elevated success rates on the long-term treatment in the last ten years. However, the risk of peri-implantitis and implant failure is the main complication of implantology. The presence of a micro gap at the implant-abutment connection (IAC) allows microorganisms to penetrate and colonize the inner part of the implant leading to biofilm accumulation and consequently to peri-implantitis development. Some chemical devices (CD) has been studied to reduce bacterial penetration at IAC level but no one ha...
Source: Journal of Biological Regulators and Homeostatic Agents - Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research
Publication date: January 2020Source: LWT, Volume 117Author(s): Haiying Cui, Chenghui Zhang, Changzhu Li, Lin LinAbstractEscherichia coli O157:H7 (E. coli O157:H7) easily forms biofilms on pores and veins of plant, which poses a serious hazard to safety of ready-to-eat vegetables. Therefore, this study was designed to evaluate inhibition mechanism of clove oil on E. coli O157:H7 biofilm and apply it to vegetable for reduce risk of contamination. The results of crystal violet staining showed that clove oil could effectively remove biofilm. After treatment of biofilm with clove oil (2 MBIC), the results of resazurin method s...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Emerging evidence suggests that altered intestinal microbiota plays an important role in the pathogenesis of many liver diseases, mainly by promoting inflammation via the “intestinal microbiota-immunity-liver” axis. We aimed to investigate the fecal microbiome of liver recipients with abnormal/normal liver function using 16S rRNA gene sequencing. Fecal samples were collected from 90 liver recipients (42 with abnormal liver function (Group LT_A) and 48 with normal liver function (Group LT_N)) and 61 age- and gender-matched healthy controls (HCs). Fecal microbiomes were analyzed for comparative composition, diver...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Smith Etareri Evivie1,2†, Amro Abdelazez1,3, Bailiang Li1†, Xin Bian4, Wan Li1, Jincheng Du1, Guicheng Huo1* and Fei Liu1 1Key Laboratory of Dairy Science, Ministry of Education, College of Food Sciences, Northeast Agricultural University, Harbin, China 2Food Science and Nutrition Unit, Department of Animal Science, Faculty of Agriculture, University of Benin, Benin City, Nigeria 3Department of Dairy Microbiology, Animal Production Research Institute, Agriculture Research Center, Giza, Egypt 4Department of Food Engineering, Harbin University of Commerce, Harbin, China Foodborne pathogens are a ma...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
ConclusionsOur study indicated that B-HCC and NBNC-HCC patients showed differential abundance of bacteria involved in different functions or biological pathways. We suggested the modification of specific gut microbiota may provide the therapeutic benefit for B-HCC and NBNC-HCC.
Source: Gut Pathogens - Category: Microbiology Source Type: research
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