Apoptotic markers and DNA damage are related to late phase of stroke: Involvement of dyslipidemia and inflammation

Publication date: 1 November 2015 Source:Physiology & Behavior, Volume 151 Author(s): Eduardo Tanuri Pascotini, Ariane Ethur Flores, Aline Kegler, Patricia Gabbi, Guilherme Vargas Bochi, Thais Doeler Algarve, Ana Lucia Cervi Prado, Marta M.M.F. Duarte, Ivana B.M. da Cruz, Rafael Noal Moresco, Luiz Fernando Freire Royes, Michele Rechia Fighera Oxidative stress and brain inflammation are thought to contribute to the pathophysiology of cerebral injury in acute stroke, leading to apoptosis and cell death. Lipid accumulation may lead to progression of carotid plaques and inflammation, contributing to increased acute stroke risk. However, little is known about these events and markers in the late stroke (>6months) and if dyslipidemia could contribute to disease's pathophysiology in a later phase. In this case–control study, we recruited patients in the late stroke phase (n=40) and health subjects (control group; n=40). Dichlorodihydrofluorescein (DCFH), nitrite/nitrate (NOx), Tumor necrosis factor-alpha (TNF-α), Acetylcholinesterase (AChE), Caspase 8 (CASP 8), Caspase 3 (CASP 3) and Picogreen (PG) were measured in periphery blood samples. Furthermore, a correlation among all measured markers (DCFH, NOx, TNF-α, AChE, CASP 8, CASP 3 and PG) was realized. The marker levels were also compared to triglycerides (TG), total (CHO), LDL and HDL cholesterol levels and medications used. Statistical analyses showed that stroke patients presented a...
Source: Physiology and Behavior - Category: Physiology Source Type: research