Toxic effects of aniline in liver, gills and kidney of freshwater fish Channa punctatus after acute exposure

Comp Biochem Physiol C Toxicol Pharmacol. 2024 Apr 8:109916. doi: 10.1016/j.cbpc.2024.109916. Online ahead of print.ABSTRACTAniline (C6H5NH2) is one of the hazardous aromatic amine where an amino group (-NH2) is connected to phenyl ring (C6H5). Based on the evaluation of the 96-hour LC50 of aniline, two sublethal concentrations (4.19 mg/l and 8.39 mg/l) were selected for acute exposure tests in freshwater fish Channa punctatus. The liver, gills and kidney of fish being the principal sites of xenobiotic material accumulation, respiration, biotransformation, and excretion are the focus of the present study. Throughout the exposure time, the comet assay revealed increased tail length and tail DNA percentage indicating maximum damage to liver, gills and kidney of treated group after 96 h. After acute exposure, there was a significant (p ≤ 0.05) increase in the enzymatic activity of glutathione-S-transferase (GST) and acetylcholinesterase (AChE), whereas decline in superoxide dismutase (SOD) and catalase (CAT) activity was observed. Meanwhile, levels of malondialdehyde (MDA) increased over the exposure period for both concentrations. After 96 h of exposure, degree of tissue change (DTC) was evaluated in liver, gill and kidney of aniline exposed fish. Additionally, light microscopy revealed multiple abnormalities in liver, gills and kidney of all the treated groups. Significant changes were observed in the levels of biochemical markers viz., glucose, triglyceride, cholesterol, as...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - Category: Biochemistry Authors: Source Type: research