Proteomics on human cerebral cavernous malformations reveals novel biomarkers in neurovascular dysfunction for the disease pathology

Biochim Biophys Acta Mol Basis Dis. 2024 Mar 25:167139. doi: 10.1016/j.bbadis.2024.167139. Online ahead of print.ABSTRACTBACKGROUND: Cerebral cavernous malformation (CCM) is a disease associated with elevated risk of focal neurological deficits, seizures, and hemorrhagic stroke. The disease has an inflammation profile and improved knowledge of CCM pathology mechanisms and exploration of candidate biomarkers will enable new non-invasive treatments.METHODS: We analyzed protein expression signature in human CCM tissue samples by using a highly specific and sensitive multiplexing technique, proximity extension assay.FINDINGS: Data analysis revealed CCM specific proteins involved in endothelial dysfunction/inflammatory activation, leukocyte infiltration/chemotaxis, hemostasis, extracellular matrix dysfunction, astrocyte and microglial cell activation. Biomarker expression profiles matched bleeding status, especially with higher levels of inflammatory markers and activated astrocytes in ruptured than non-ruptured samples, some of these biomarkers are secreted into blood or urine. Furthermore, analysis was also done in a spatially resolving manner by separating the lesion area from the surrounding brain tissue. Our spatial studies revealed that although appearing histologically normal, the CCM border areas had become pathological when compared to control brain tissues. Moreover, the functional relevance of CD93, ICAM-1 and MMP9, markers related to endothelial cell activation and ext...
Source: Biochimica et Biophysica Acta - Category: Biochemistry Authors: Source Type: research