DNA Methylation of a Group of Long Non-Coding RNA Genes at Different Stages of Ovarian Cancer Dissemination

There are three types of metastases in ovarian cancer: lymphogenous, hematogenous, and peritoneal. Dissemination of the tumor in the peritoneum is directly related with the development of ascites and a poor prognosis. The purpose of this study is to determine changes in the methylation level of a group of long non-coding RNA (lncRNA) genes at different stages of ovarian cancer progression. The methylation level of 7 lncRNA genes (LINC00472,LINC00886,MAFG-DT,SNHG1,SNHG6,TP53TG1, andTUG1) was studied by quantitative methyl-specific PCR in 93 samples of ovarian tumors and 75 paired samples of histologically normal tissue, as well as in 29 peritoneal macroscopic metastases. Using the nonparametric Mann —Whitney test, a significant (p<0.001) increase in the level of methylation of theLINC00886,SNHG1,SNHG6, andTUG1 genes in the tumor tissue was shown. For theLINC00472,LINC00886, andSNHG6 genes, a significant relationship was found with the clinical stage (p≤0.001), as well as with the appearance of metastases for theLINC00472 (p<0.001) andSNHG6 (p=0.005) genes. There was a significant increase in the level of methylation ofMAFG-DT andTP53TG1 (p<0.001) genes, as well as a decrease inLINC00886 (p=0.003) in peritoneal metastases relative to the primary focus. Methylation of theLINC00472 andSNHG6 genes can be considered as a factor in initiating ovarian cancer metastasis, and methylation of theLINC00886,MAFG-DT, andTP53TG1 genes as a colonization factor for metastases in t...
Source: Bulletin of Experimental Biology and Medicine - Category: Biology Source Type: research