Viruses, Vol. 16, Pages 438: Novel Universal Recombinant Rotavirus a Vaccine Candidate: Evaluation of Immunological Properties

Viruses, Vol. 16, Pages 438: Novel Universal Recombinant Rotavirus a Vaccine Candidate: Evaluation of Immunological Properties Viruses doi: 10.3390/v16030438 Authors: Dmitriy L. Granovskiy Nelli S. Khudainazarova Ekaterina A. Evtushenko Ekaterina M. Ryabchevskaya Olga A. Kondakova Marina V. Arkhipenko Marina V. Kovrizhko Elena P. Kolpakova Tatyana I. Tverdokhlebova Nikolai A. Nikitin Olga V. Karpova Rotavirus infection is a leading cause of severe dehydrating gastroenteritis in children under 5 years of age. Although rotavirus-associated mortality has decreased considerably because of the introduction of the worldwide rotavirus vaccination, the global burden of rotavirus-associated gastroenteritis remains high. Current vaccines have a number of disadvantages; therefore, there is a need for innovative approaches in rotavirus vaccine development. In the current study, a universal recombinant rotavirus antigen (URRA) for a novel recombinant vaccine candidate against rotavirus A was obtained and characterised. This antigen included sequences of the VP8* subunit of rotavirus spike protein VP4. For the URRA, for the first time, two approaches were implemented simultaneously—the application of a highly conserved neutralising epitope and the use of the consensus of the extended protein’s fragment. The recognition of URRA by antisera to patient-derived field rotavirus isolates was proven. Plant virus-based spherical particles (SP...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research