Approaches to probe and perturb long noncoding RNA functions in diseases

Curr Opin Genet Dev. 2024 Feb 26;85:102158. doi: 10.1016/j.gde.2024.102158. Online ahead of print.ABSTRACTLong noncoding RNAs (lncRNAs) are a class of RNA molecules exceeding 200 nucleotides in length that lack long open-reading frames. Transcribed predominantly by RNA polymerase II (>500nt), lncRNAs can undergo splicing and are produced from various regions of the genome, including intergenic regions, introns, and in antisense orientation to protein-coding genes. Aberrations in lncRNA expression or function have been associated with a wide variety of diseases, including cancer, cardiovascular diseases, diabetes, and neurodegeneration. Despite the growing recognition of select lncRNAs as key players in cellular processes and diseases, several challenges obscure a comprehensive understanding of their functional landscape. Recent technological innovations, such as in sequencing, affinity-based techniques, imaging, and RNA perturbation, have advanced functional characterization and mechanistic understanding of disease-associated lncRNAs.PMID:38412563 | DOI:10.1016/j.gde.2024.102158
Source: Current Opinion in Genetics and Development - Category: Genetics & Stem Cells Authors: Source Type: research