Analysis of Notch1 protein expression in methotrexate-associated lymphoproliferative disorders

In this study, to investigate the association of NOTCH1 in the pathogenesis of MTX-LPD, we evaluated protein expression of Notch1 in nuclei immunohistochemically in MTX-LPD cases [histologically DLBCL-type (n = 24) and classical Hodgkin lymphoma (CHL)-type (n = 24)] and de novo lymphoma cases [DLBCL (n = 19) and CHL (n = 15)]. The results showed that among MTX-LPD cases, the expression of Notch1 protein was significantly higher in the DLBCL type than in the CHL type (P < 0.001). In addition, among DLBCL morphology cases, expression of Notch1 tended to be higher in MTX-LPD than in the de novo group; however this difference was not significant (P = 0.0605). The results showed that NOTCH1 may be involved in the proliferation and tumorigenesis of B cells under the use of MTX. Further research, including genetic studies, is necessary.PMID:38281745 | DOI:10.3960/jslrt.23038

Source: Journal of Clinical and Experimental Hematopathology : JCEH - January 28, 2024 Category: Hematology Authors: Takeshi Okatani Midori Filiz Nishimura Yuria Egusa Sayako Yoshida Yoshito Nishimura Asami Nishikori Tadashi Yoshino Hidetaka Yamamoto Yasuharu Sato Source Type: research