AsOBP1 is required for bioallethrin repellency in the malaria vector mosquito Anopheles sinensis

Bioallethrin, the first commercially available volatile pyrethroid, has been used worldwide in insecticides and repellents. The olfactory mechanism of the repellency elicited by bioallethrin in mosquitoes is still unclear. The binding ability of recombinantAsOBP1 protein to bioallethrin and the reduced bioallethrin repellency and electroantennogram responses inAsOBP1−/− mutants demonstrated that theAsOBP1-mediated olfactory pathway is an important component of bioallethrin repellency inAnopheles sinensis. Moreover, the reduced bioallethrin repellency in pyrethroid-resistant mosquitoes may be related tokdr mutation and reduced olfactory sensitivity. AbstractThe use of insecticides, primarily pyrethroids, is a pivotal strategy for mosquito control globally. Bioallethrin, the first commercially available volatile pyrethroid, can elicit spatial (i.e., noncontact) repellency to mosquitoes through the coactivation of olfactory receptor neurons and sodium channels. However, the olfactory mechanism of the repellency elicited by bioallethrin in mosquitoes is still unclear. Here, we demonstrated the involvement ofAsOBP1 in the bioallethrin repellency inAnopheles sinensis, one of the main vectors of vivax malaria in China and other Southeast Asian countries. The behavioral and electrophysiological analyses inAsOrco−/− mutant found that the spatial repellency elicited by bioallethrin depended on the odorant receptor (OR)-mediated olfactory pathway. Furthermore, the repellency was...

Source: Insect Science - February 23, 2024 Category: Biology Authors: Yongjie Zhang, Shulin He, Chengyin He, Ling Zhou, Ou Xu, Liang Qiao, Bin Chen, Yueqing Cao, Zhengbo He Tags: ORIGINAL ARTICLE Source Type: research