Synthesis of Ciprofloxacin-Conjugated Gold Nanoparticles and their Study Antibacterial Effects on Growth Biofilm Formation Through Nebulizer Mask Against Respiratory Infection

AbstractAn efficient strategy involves the use of antibiotic-conjugated nanoparticles. This research primarily aimed to enhance the biosynthesis of gold nanoparticles by binding them with ciprofloxacin. We investigated the biological, optical, and structural characteristics of gold nanoparticles (AuNPs) and their combination with ciprofloxacin (CIP@AuNPs). We performed a number of analyses using Fourier-transform infrared (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and UV –VIS spectroscopy using a cost-effective pulsed-laser ablation technique (PLAL). The size and morphology of the nanoparticles were notably determined by the TEM analysis, which showed an average particle size of less than 20 nm. We also investigated the antibacterial effects of AuNPs, ciprofloxac in, and CIP@AuNPs against two bacterial strains: the gram-negativeKlebsiella pneumonia and the gram-positiveStreptococcus pneumonia. Utilizing the well-diffusion method, we observed that CIP@AuNPs exhibited greater effectiveness compared to ciprofloxacin alone or AuNPs alone. The bacterial strains tested in this study exhibited a high degree of resistance to the antibiotics under investigation. The antimicrobial activity assay revealed the most significant inhibition zone when dealing withS. pneumonia, as compared toK. pneumonia. Notably, Ciprofloxacin-conjugated gold nanoparticles displayed a more pronounced inhibitory effect when contrasted with Ciprofloxacin or standalone AuNPs. We...
Source: Plasmonics - Category: Biomedical Science Source Type: research