Deep sequencing reveals recurrent somatic mutations and distinct molecular subgroups in gastric cancer in Mizo population, North East India

Genomics. 2023 Nov 13:110741. doi: 10.1016/j.ygeno.2023.110741. Online ahead of print.ABSTRACTIn India, Mizoram has the highest incidence of gastric cancer (GC) which might be associated with environmental factors such as diet, Helicobacter pylori (H.pylori) and Epstein-Barr virus (EBV) infections, and somatic genomic alterations. We performed PCR cum sequencing and fragment analysis for detection of H. pylori/EBV infection and microsatellite Instability (MSI) in GC patients (N = 68). Somatic mutations were identified by targeted and exome sequencing. We found 87% of GC patients infected with H. pylori and EBV. Pathogenic infections were mostly mutually exclusive with only 16% of coinfection. TP53, MUC6, and ARID1A were significantly mutated. Two molecular subgroups with distinctive mutational profiles were identified: (1) patients harboring mutations in TP53 and (2) patients harboring mutations in RTK/RAS/PI3-K signaling pathway and chromatin-remodeling genes. Therefore, EBV and H. pylori infections and somatic mutations in the genes involved in RTK/RAS/PI3K signaling pathway, chromatin-remodeling, and TP53 might drive GC development and progression in Mizo patients.PMID:37967684 | DOI:10.1016/j.ygeno.2023.110741

Source: Genomics - November 15, 2023 Category: Genetics & Stem Cells Authors: Payel Chakraborty Sillarine Kurkalang Souvik Ghatak Subrata Das Arindam Palodhi Sumanta Sarkar Ranjan Dhar Saia Chenkual Lalawmpuii Pachuau John Zohmingthanga Jeremy L Pautu Thomas Zomuana Sailo Tlau Lalruatfela John Zothanzama Nachimuthu Senthil Kumar Ar Source Type: research