Isolation and propagation of bovine blood-derived macrophages using a mixed culture with bovine endothelial B46 cells

In conclusion, this study provides a simple and efficient protocol for the isolation and propagation of BBMs using a primary mixed culture of bovine whole blood with B46 feeder cells. Isolated BBMs are expected to be useful for developing in vitro models for studying the interactions between bovine pathogens and host immune cells.PMID:37920877 | DOI:10.1002/cbin.12102

Source: Cell Biology International - November 3, 2023 Category: Cytology Authors: Kanae Hiramatsu Rina Ikeda Satoko Kawaji Yuichi Ueno Reiko Nagata Ken-Go Hayashi Kosuke Iga Miyako Yoshioka Takato Takenouchi Source Type: research