iTRAQ-based proteomics identifies proteins associated with betaine accumulation in Lycium barbarum L

J Proteomics. 2023 Oct 23:105033. doi: 10.1016/j.jprot.2023.105033. Online ahead of print.ABSTRACTIn order to better understand the mechanism of betaine accumulation in Lycium barbarum L. (LBL), we used iTRAQ (Isotope relative and absolute quantitative labeling) proteomics to screen and identify differentially expressed proteins (DEPs) at five stages (S1-young fruit stage, S2-green fruit stage, S3- early yellowing stage, S4-late yellowing stage, S5-ripening stage). A total of 1799 DEPs and 171 betaine-related DEPs were identified, and phosphatidylethanolamine N-methyltransferase (NMT), choline monooxygenase (CMO), and betaine aldehyde dehydrogenase (BADH) were found to be the key enzymes related to betaine metabolism. These proteins are mainly involved in carbohydrates, amino acids and their derivatives, fatty acids, carboxylic acids, photosynthesis and photoprotection, isoquinoline alkaloid biosynthesis, peroxisomes, and glycine, serine, and threonine metabolism. Three of the key enzymes were also up- and down-regulated to different degrees at the mRNA level. The study provide new insights into the of mechanism of betaine accumulation in LBL. SIGNIFICANCE: Betaine, a class of naturally occurring, water-soluble alkaloids, has been found to be widespread in animals, higher plants, and microbes. In addition to being an osmotic agent, betaine has biological functions such as hepatoprotection, neuroprotection, and antioxidant activity. Betaine metabolism (synthesis and catabolism...
Source: Journal of Proteomics - Category: Biochemistry Authors: Source Type: research