Epithelial metabolic stress in IPF: Identifying intervention strategies using a preclinical murine SftpcI73T model of lung fibrosis

Disruptions to autophagy, mitochondrial biology, and glycolysis are recognized features of alveolar type 2 (AT2) cell stress associated with human IPF. We aimed to leverage our published inducible murine SftpcI73T (IER-SP-CI73T) model of lung fibrosis to catalogue AT2 metabolic dysfunction, identify therapeutic targets, and gauge effect size from focused rescue of the disease phenotype. Primary SftpcI73T AT2 cells (AT2I73T) exhibit acquired autophagic defects that coincide with transcriptomic upregulation in glucose utilization / carbohydrate metabolism pathways and functional biochemical increases in glucose flux and extracellular lactate. In "Seahorse" assays, AT2I73T isolated 2- and 4-weeks post-induction show progressive impairment in respiration along with decreased (depolarized) mitochondrial transmembrane potential (), disruption of mitochondrial networks (increased fission), and a concurrent decrease in mitochondrial biogenesis. AT2I73T bioenergetic changes were associated with increased AT2 cellular ATP content and inhibition of the AMPK-PPAR signaling hub. In primary AT2I73T, AMPK agonism (PF06409577) or stimulation of PGC1α (Rosiglitazone), but not mTOR inhibition (Rapamycin) rescued mitochondrial respiration. In vivo proof of concept was done using Metformin (indirect AMPK agonist) in preventative or therapeutic protocols. Delivered once daily to SftpcI73T mice, Metformin reduced mortality, BAL protein, and BAL soluble collagen while improving static lung co...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Mechanisms of lung injury and repair Source Type: research