Responses of digestive enzyme profiles in newly-hatched (Zoea I) larvae of the mud crab Scylla serrata to intermittent food availability and food deprivation

Comp Biochem Physiol B Biochem Mol Biol. 2023 Oct 8:110906. doi: 10.1016/j.cbpb.2023.110906. Online ahead of print.ABSTRACTActivities (mU larva-1) of enzymes critical to digestion were examined to better understand how newly-hatched (Zoea I) larvae of the mud crab Scylla serrata respond to intermittent food availability and food deprivation. Specifically, this study examined the activities of trypsin-like proteases, nonspecific esterases, and α-amylase across three experiments that simulated scenarios in which larvae hatch and experience rearing conditions where food was either: (1) continuously available or unavailable; (2) initially unavailable, but subsequently available; or (3) initially available, but subsequently unavailable. Results showed that food availability exerts a significant influence on enzyme profiles in newly-hatched larvae, with nutritional history influencing their response to food deprivation. When food was unavailable from hatch, there was no significant change in larval enzyme activities between 6 and 78 h post-hatch. If food became available at any point during this period, however, newly-hatched larvae were capable of rapidly (within 12-24 h) adjusting enzyme activities in response. Furthermore, a short (36 h) period of food availability appears sufficient to permit continuous substrate utilization during subsequent food deprivation of equivalent duration. Such flexibility is an important physiological strategy allowing newly-hatched larvae of S. ser...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - Category: Biochemistry Authors: Source Type: research