Photobiomodulation therapy enhances neural differentiation of dental pulp stem cells via ERK1/2 signaling pathway

This study demonstrated that photobiomodulation therapy (PBMT) could promote the neural differentiation of DPSCs, and the mechanism was related to the ERK1/2 signaling pathway. The results provided evidence that PBMT could assist in the transplantation of DPSCs in the treatment of nervous diseases. AbstractPhotobiomodulation therapy (PBMT) is the application of a low-level laser device to generate physiological changes and provide therapeutic effects. Till now, the effects of PBMT on the neural differentiation of mesenchymal stem cells have been rarely reported. Herein, the potential effect and mechanism of PBMT on the neural differentiation of dental pulp stem cells (DPSCs) were preliminarily investigated in our research. The optimal dose of 3.75  J/cm2 was first screened for use in the following neural-inducing studies. Then, DPSCs were cultured in neural induction medium and treated with laser irradiation for 7  days. From the results of morphology and immunofluorescence, we found that irradiation promoted the formation of neural stem cell-like spheroids derived from DPSCs and enhanced potential neural differentiation. Furthermore, neural differentiation gene expressions of Nestin, microtubule-associated protein-2, and neural cell adhesion molecule were increased after PBMT irradiation. The protein expressions of class III β-tubulin and neurogenic differentiation factor 1 were also improved. Meanwhile, the involvement of extracellular signal-regulated kinase (ERK1/2) ...
Source: Photochemistry and Photobiology - Category: Science Authors: Tags: RESEARCH ARTICLE Source Type: research