Larvicidal activity of green synthesized iron oxide nanoparticles using Grevillea robusta Cunn. leaf extract against vector mosquitoes and their characterization

Exp Parasitol. 2023 Jul 17:108586. doi: 10.1016/j.exppara.2023.108586. Online ahead of print.ABSTRACTExtensive fumigation of synthetic pesticides to control the mosquito vector during each post-monsoon season in Pakistan significantly enhanced the environmental contamination and extinction of beneficial insects from the urban ecosystems. In this context, the present study examined the larvicidal efficacy of green synthesized iron nanoparticles (IONPs), using an aqueous leaf extract of Grevillea robusta against the early 2nd and 4th instar larvae of Aedes aegypti and Anopheles stephensi in Pakistan. The prepared IONPs were characterized by UV-Vis spectrum, FTIR, X-ray diffraction, scanning electron microscopy, and energy-dispersive diffraction. Larvicidal bioassay was conducted at various concentrations (80, 160, 240, 320, and 400 ppm) of IONPs prepared from leaf extract of G. robusta, and readings were taken-every 12 h for two consecutive days. In vitro, larvicidal assay, G. robusta leaf extract IONPs exhibited high mortalities of 64-96% (LC50 = 259.07 ppm; LC90 = 443.92 ppm) for the second instar and 65-98% (LC50 = 238.05 ppm; LC90 = 433.93 ppm) for the fourth instar of Ae. aegypti, while in the case of An. stephensi 56-84% (LC50 = 297.96 ppm; LC90 = 528.69 ppm) for the second and 56-88% (LC50 = 292.72 ppm; LC90 = 514.00 ppm) mortality for fourth larvae at 12-48 h post-exposure times were observed respectively. Significant (p < 0.05) dose-dependent and exposure time-depen...
Source: Experimental Parasitology - Category: Parasitology Authors: Source Type: research