Short-term swimming up-regulates pro-inflammatory mediators and cytokines in gilthead seabream (Sparus aurata)

Comp Biochem Physiol A Mol Integr Physiol. 2023 Jul 10;284:111487. doi: 10.1016/j.cbpa.2023.111487. Online ahead of print.ABSTRACTAerobic swimming exercise in fish has been shown to improve robustness of some species. However, the optimal conditions to be applied and the mechanisms underlying remain unknown. We investigated the effects of 6 h of induced swimming on the immune response of gilthead seabream (Sparus aurata), by analysing markers related to immune status in plasma, skin mucus, gills, heart and head-kidney. Forty fish were individually exercised in swim tunnels by applying different water currents: steady low (SL, 0.8 body lengths (BL) s-1), steady high (SH, 2.3 BL s-1), oscillating low (OL, 0.2/0.8 BL s-1) and oscillating high (OH, 0.8/2.3 BL s-1) velocities, including a non-exercised group with minimal water flow (MF, <0.1 BL s-1). Swimming conditions did not trigger a stress response or anaerobic metabolism, suggested by similar levels of cortisol, lactate, and glucose in plasma among groups. Blood haemoglobin and innate immune parameters in plasma and skin mucus also remained unaltered. However, decreased blood haematocrit was observed in fish swimming on the OL condition. Interestingly, gene expression analysis revealed that the OL condition led to the up-regulation of pro-inflammatory mediators (nfκb1 and mapk3) and cytokines (tnfα, il1β and il6) in gills. A similar response occurred in heart, with an up-regulation of nfκb1, tnfα, il6 and cox2 in the...
Source: Comparative Biochemistry and Physiology. Part A, Molecular and integrative physiology. - Category: Physiology Authors: Source Type: research