GSE235951 Effect of chemotherapy on the uterus of young adult cancer survivors

Contributors : Tim Jenkins ; Deepika Garg ; Matthew Hodgman ; Sydney Reil ; Lesley Lomo ; Kenneth I Aston ; Jonathon Hill ; Erica Johnstone ; Joseph M LetourneauSeries Type : Methylation profiling by genome tiling arrayOrganism : Homo sapiensThis pilot study aimed to accomplish 3 distinct but related goals. First, we described the structural phenotype of uterine/endometrial injury after alkylating chemotherapy sonographically and histologically. Second, we investigated the molecular impact of alkylating chemotherapy exposure on messenger RNA expression and epigenetic changes in endometrial biopsy (EMB) tissue homogenates. Third, we investigated the feasibility of performing ultrasonographic and EMB-based assessments in reproductive-age cancer survivors. Because of limited information available in this area of inquiry, this approach is exploratory and should prove to be hypothesis-generating. The main outcome measures of this study included endometrial thickness (EMT) and uterine volume on transvaginal ultrasound. Endometrial histology, DNA methylation, and RNA-seq on EMB samples, and pain scale assessment before and after EMB.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Methylation profiling by genome tiling array Homo sapiens Source Type: research