Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants

In this study, based on an existing reverse transcriptase real-time PCR (RT-qPCR) assay, we developed a reverse transcriptase droplet digital PCR assay (RT-ddPCR) to detect and quantify the frequency of the E119V-NA mutation. Furthermore, reverse genetics viruses carrying this mutation were created to test the performance of the RT-ddPCR assay and compare it to the standard phenotypic NA assay. We also discuss the advantage of using an RT-ddPCR instead of qPCR method in the context of viral diagnostics and surveillance.PMID:36975535 | PMC:PMC10047791 | DOI:10.3390/cimb45030165

Source: Current Issues in Molecular Biology - March 28, 2023 Category: Molecular Biology Authors: Laura A E Van Poelvoorde Fran çois E Dufrasne Steven Van Gucht Xavier Saelens Nancy H C Roosens Source Type: research