Loss of Nf1 and Ink4a/Arf Are Associated with Sex-Dependent Growth Differences in a Mouse Model of Embryonal Rhabdomyosarcoma

This study describes a new model of embryonal rhabdomyosarcoma driven by the loss of Nf1 and Ink4a/Arf, two mutations commonly found in patient tumors. We find that this new model is histologically similar to other previously-published rhabdomyosarcoma models, although it substantially differs in the time required for tumor onset and in tumor growth kinetics. We also observe unique sex-dependent phenotypes in both primary and newly-developed orthotopic syngeneic allograft tumors that are not present in previous models. Using in vitro and in vivo studies, we examined the response to vincristine, a component of the standard-of-care chemotherapy for RMS. The findings from this study provide valuable insight into a new mouse model of rhabdomyosarcoma that addresses an ongoing need for patient-relevant animal models to further translational research.PMID:36826025 | PMC:PMC9955904 | DOI:10.3390/cimb45020080

Source: Current Issues in Molecular Biology - February 24, 2023 Category: Molecular Biology Authors: Wade R Gutierrez Jeffrey D Rytlewski Amanda Scherer Grace A Roughton Nina C Carnevale Krisha Y Vyas Gavin R McGivney Qierra R Brockman Vickie Knepper-Adrian Rebecca D Dodd Source Type: research