Psidium guajava induces cytotoxicity in human malignant glioblastoma cell line: Role of reactive oxygen species

Toxicol In Vitro. 2023 Feb 8;89:105567. doi: 10.1016/j.tiv.2023.105567. Online ahead of print.ABSTRACTOne of the deadliest types of CNS primary brain cancers is glioblastoma multiforme (GBM), and the survival rate of patients is about 7.2%. The standard treatment for GBM is surgical interventions followed by temozolomide. We investigated for the first time, the cytotoxic impacts of Psidium guajava (P. guajava) on the U87 GBM cell line. We measured cell toxicity through the MTT test following 24 h, 48 h, and 72 h treatment with different concentrations of fruit and seed hydroalcoholic extracts of P. guajava (25-400 μg/ml). Lipid peroxidation assay, reactive oxygen species (ROS) production, and apoptosis rate were evaluated 24 h after treatment by extracts of P. guajava. Moreover, to determine the Bax/Bcl-2 and NF-κB genes expression, we performed a real-time polymerase chain reaction (RT-PCR). Our finding demonstrated that 50-400 μg/ml of P. guajava extracts dose-dependently decreased the viability of U87 cells. Also, treatment by extracts increased lipid peroxidation, ROS production, and apoptosis in a dose-dependent manner. Moreover, the RT-PCR demonstrated an up-regulation in Bax\Bcl-2 and NF-κB. Thus, P. guajava inhibited the proliferation of U87 GBM cells and increased apoptosis probably through Bax/Bcl-2 and NF-κB regulation.PMID:36758825 | DOI:10.1016/j.tiv.2023.105567

Source: Toxicology in Vitro - February 9, 2023 Category: Toxicology Authors: Azar Hosseini Mohaddeseh Sadat Alavi Amir Reza Shahraki Arezoo Rajabian Amir Reza Afshari Prashant Kesharwani Amirhossein Sahebkar Source Type: research