Combining povidone ‐iodine with vancomycin can be beneficial in reducing early biofilm formation of methicillin‐resistant Staphylococcus aureus and methicillin‐sensitive S. aureus on titanium surface

The objectives of this in vitro study were to test the efficacy of povidone-iodine (PVP-I) and vancomycin in treating premature and developedStaphylococcus aureus biofilms grown on titanium implant surfaces. PVP-I and vancomycin were used to treat immature and developed biofilms formed by two clinical strains ofS. aureus (BP043-MRSA, PB011-MSSA).S. aureus strains were grown as immature (3  h-old) or developed (24 h-old) biofilm. These biofilms were grown on titanium plasma sprayed discs. The treatment regimens tested were: 0.8% PVP-I, 500 μg/ml vancomycin as well as a combination of vancomycin and PVP-I. PVP-I was tested at 3 min, as per current clinical practice, versus 1 mi n treatment times. In addition, the cytotoxicity of the PVP-I and vancomycin was tested using fresh skeletal muscle tissue cores harvested from the rat's abdominal muscles using alamarBlue assay. The combination of PVP-I (3 min) and vancomycin (24 h.) showed synergistic interaction and the best e fficacy against immature biofilms formed by both clinical strains. This degree of eradication was statistically significant compared to the untreated control,p <  .0001. However, this combination therapy had limited efficiency against developed biofilms. Also, PVP-I alone was more effective when exposure time was 3 min instead of 1 min against immature biofilm for MRSA,p = .02, and MSSA,p = .01. PVP-I and vancomycin were not effective against developed biofilm regardless of exposure time....
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - Category: Materials Science Authors: Tags: RESEARCH ARTICLE Source Type: research