Curcumin-Loaded Chitosan Nanoparticle Preparation and Its Protective Effect on Celecoxib-induced Toxicity in Rat isolated Cardiomyocytes and Mitochondria
Drug Res (Stuttg) DOI: 10.1055/a-1960-3092Curcumin has a wide range of pharmacological activities, including antioxidant,
anti-inflammatory and tissue protective. In here we hypothesized that
curcumin-loaded chitosan-coated solid lipid nanoparticles (CuCsSLN) are able to
increase its overall bioavailability and hence its antioxidant and
mitochondria;/lysosomal protective properties of curcumin. CuCsSLN were
prepared using solvent diffusion technique for formation of solid lipid
nanoparticles (SLNs) and electrostatic coating of positive-charged chitosan to
negative surface of SLNs. CuCsSLN showed the encapsulation efficiency of
91.4±2.7%, the mean particle size of 208±9 nm,
the polydispersity index of 0.34±0.07, and the zeta potential
of+53.5±3.7 mV. The scanning electron microscope (SEM)
images of nanoparticles verified their nanometric size and also spherical shape.
Curcumin was released from CuCsSLN in a sustain release pattern up to
24 hours. Then isolated cardiomyocytes and mitochondria were
simultaneously treated with (1) control (0.05% ethanol), (2) celecoxib
(20 µg/ml) treatment, (3) celecoxib
(20 µg/ml)+++CuCsSLN
(1 µg/ml) treatment, (4) CuCsSLN
(1 µg/ml) treatment, (5) celecoxib
(20 µg/ml)+++curcumin
(10 µM) treatment and (6) curcumin (10 µ...
Source: Drug Research - Category: Drugs & Pharmacology Authors: Ebrahimi, Hossein Ali Esmaeli, Samira Khezri, Saleh Salimi, Ahmad Tags: Original Article Source Type: research
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